Melbourne/Lab BL Notebook/PCR1
From 2007.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII. | For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII. | ||
| - | ====PCR mix==== | + | |- |
| - | 5ul 10x buffer\\ | + | ====PCR mix==== |
| + | |5ul 10x buffer\\ | ||
5ul Enhancer | 5ul Enhancer | ||
| Line 24: | Line 25: | ||
50ul Total | 50ul Total | ||
| + | | | ||
| + | |- | ||
Revision as of 14:51, 10 October 2007
Protocol for PCR reactions A~G
For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
|-
PCR mix
|5ul 10x buffer\\
5ul Enhancer
0.6ul dNTPs (25mM stock)
2.5ul MgSO4 (Supplied in PCR kit)
1.5ul Primer I (10uM)
1.5ul Primer II (10uM)
1ul Template (pJS010)
0.4ul Pfx Platinum (Invitrogen)
32.5ul ddH2O
---
50ul Total | |-
