Melbourne/Lab BL Notebook/PCR1
From 2007.igem.org
(Difference between revisions)
| Line 25: | Line 25: | ||
32.5ul ddH<sub>2</sub>O | 32.5ul ddH<sub>2</sub>O | ||
|94°C | |94°C | ||
| + | 94°C | ||
| + | 59°C | ||
| + | 68°C | ||
| + | 68°C | ||
| + | 4°C <math>\infty</math> | ||
|- | |- | ||
Revision as of 14:59, 10 October 2007
Protocol for PCR reactions A~G
For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
| PCR mix | PCR program |
|---|---|
| 5ul 10x buffer\\
5ul Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer I (10uM) 1.5ul Primer II (10uM) 1ul Template (pJS010) 0.4ul Pfx Platinum (Invitrogen) 32.5ul ddH2O | 94°C
94°C 59°C 68°C 68°C 4°C <math>\infty</math> |
| 50ul Total |
|-
PCR mix
|5ul 10x buffer\\
5ul Enhancer
0.6ul dNTPs (25mM stock)
2.5ul MgSO4 (Supplied in PCR kit)
1.5ul Primer I (10uM)
1.5ul Primer II (10uM)
1ul Template (pJS010)
0.4ul Pfx Platinum (Invitrogen)
32.5ul ddH2O
---
50ul Total | |-
