Week 15
From 2007.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
::-I763007+pSB4A3. | ::-I763007+pSB4A3. | ||
*We inoculate: | *We inoculate: | ||
| - | ::- | + | ::-I763031 (low copy plasmid); |
| + | ::-Plac-CI-Lacy (low copy plasmid); | ||
| + | ::-I763032 (high copy plasmid). | ||
::'''10/09/07''' | ::'''10/09/07''' | ||
| - | + | *Transformation of: | |
| + | ::-pTet-LacI-T + I763007; | ||
| + | ::-pTet-LacI (low copy plasmid); | ||
Revision as of 09:19, 11 October 2007
- 10/08/07
- We inoculate:
- -I763031 (low copy plasmid);
- -Plac-CI-Lacy (low copy plasmid);
- -E.Coli;
- -I763032 (high copy plasmid).
- Digestion of:
- -I763035 with Eco/Pst1;
- -I763007 with Eco/spe;
- -Ptet-LacI-T with Eco/Xba;
- -pSB4A3 with Spe/Xba.
- Ligation for:
- -I763035+pSB4A3;
- -I763007+Ptet-lacI-t;
- -I763007+pSB4A3;
- -I763032+pSB4A3.
- Run on electrophoresis gel.
- I763007 and Ptet-lacI-T band extraction.
- O/N ligation for:
- -Ptet-LacI-T+I763007;
- -Ptet-LacI+pSB4A3;
- -I763032+pSB4A3;
- -I763007+pSB4A3.
- We inoculate:
- -I763031 (low copy plasmid);
- -Plac-CI-Lacy (low copy plasmid);
- -I763032 (high copy plasmid).
- 10/09/07
- Transformation of:
- -pTet-LacI-T + I763007;
- -pTet-LacI (low copy plasmid);
- 10/10/07
- 10/11/07
- 10/12/07
