Arthur Yu Notebook
From 2007.igem.org
(Difference between revisions)
(→6/4 Training Finishes, Real Stuff Starts) |
(→6/4 Training Finishes, Real Stuff Starts) |
||
| Line 9: | Line 9: | ||
* Colony PCR'd the incubated E.coli | * Colony PCR'd the incubated E.coli | ||
* Ran gel of the digest + PCR | * Ran gel of the digest + PCR | ||
| - | * PICTURE: [[Image:060407gelayu.jpg]] | + | * PICTURE: [[Image:060407gelayu.jpg|200px|right]] |
* ^ PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder | * ^ PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder | ||
* No bands for PCR or parent. Confused? Other ones look great. | * No bands for PCR or parent. Confused? Other ones look great. | ||
Revision as of 00:32, 5 June 2007
My Construction Files
My Sequencing Files
6/4 Training Finishes, Real Stuff Starts
- Incubated some colonies
- Miniprep'd already-been-incubated colonies (2)
- Double digest of the 2 minipreps + parent plasmid
- Colony PCR'd the incubated E.coli
- Ran gel of the digest + PCR
- PICTURE:
- ^ PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder
- No bands for PCR or parent. Confused? Other ones look great.
As for me: Wiki acc works now.
Designing oligos and will compare with Vai.
Ayu 18:19, 4 June 2007 (EDT)
to do
