Week 15
From 2007.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
::-I763032+pSB4A3. | ::-I763032+pSB4A3. | ||
*Run on electrophoresis gel. | *Run on electrophoresis gel. | ||
| - | *I763007 and | + | *I763007 and I763026 band extraction. |
*O/N ligation for: | *O/N ligation for: | ||
::-I763026+I763007; | ::-I763026+I763007; | ||
| Line 87: | Line 87: | ||
::-I763019; | ::-I763019; | ||
::-I763024+C0012; | ::-I763024+C0012; | ||
| - | ::- | + | ::-I763032. |
*We inoculate | *We inoculate | ||
::-I763019. | ::-I763019. | ||
Revision as of 10:24, 12 October 2007
- 10/08/07
- We inoculate:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -E.Coli;
- -I763032 (high copy plasmid).
- Digestion of:
- -I763035 with Eco/Pst1;
- -I763007 with Eco/spe;
- -I763026 with Eco/Xba;
- -pSB4A3 with Spe/Xba.
- Ligation for:
- -I763035+pSB4A3;
- -I763007+I763026;
- -I763007+pSB4A3;
- -I763032+pSB4A3.
- Run on electrophoresis gel.
- I763007 and I763026 band extraction.
- O/N ligation for:
- -I763026+I763007;
- -I763024+pSB4A3;
- -I763032+pSB4A3;
- -I763007+pSB4A3.
- We inoculate:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763032 (high copy plasmid).
- 10/09/07
- Transformation of:
- -I763026 + I763007;
- -I763024 (low copy plasmid);
- -I763032 (low copy plasmid);
- -I763007 (low copy plasmid).
- We inoculate in 6ml:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid).
- Ligation for:
- -I763016 (Eco/Spe)+GFP.(Eco/Xba).
- 10/10/07
- Miniprep of:
- -I763016 (low copy plasmid);
- -I763031(low copy plasmid).
- Digestion for :
- -I763031 with Xba/Pst1;
- -I763016 (low copy plasmid) with Spe/Pst1;
- Run on electrophoresis gel and I763016 band extraction.
- Ligation for :
- -I763016 (low copy plasmid)+GFP;
- -I763024+pSB4A3;
- -I763024+C0012(high copy plasmid).
- We inoculate in 6ml:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024(low copy plasmid).
- 10/11/07
- Stock preparation (500ul) of:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024(low copy plasmid).
- Miniprep of:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024(low copy plasmid).
- Fluorescence test of:
- -I763031 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid).
- Control digestion of:
- -I763031 with Xba/Pst1;
- -I763016 with Xba/Pst1 and Spe/Xba;
- -I763026 + I763007 with Xba/Pst1 and Spe/Xba;
- -I763024 with Xba/Pst1;
- -I763007 with Xba/Pst1.
- Run on electrophoresis gel and band extraction.
- Transformation of:
- -I763019;
- -I763024+C0012;
- -I763032.
- We inoculate
- -I763019.
- 10/12/07
- Miniprep of:
- -I763019.
- Digestion of:
- -I763019 with Xba/Pst1;
- -I763019 with Eco/Xba.
- Run on electrophoresis gel.
