BerkiGEM2007-SamanthaConstructionFile3
From 2007.igem.org
(Difference between revisions)
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'''[lox][term][lox]''' <br> | '''[lox][term][lox]''' <br> | ||
- | '''2 Different terminators (1124 and 1092 | + | '''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br> |
---- | ---- | ||
- | Digest pBca9145-I716205 (BamHI/AlwnI, | + | Digest pBca9145-I716205 (BamHI/AlwnI, 1723+553bp, Large) <br> |
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br> | Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br> | ||
Product is pBca9145-I716207. <br> | Product is pBca9145-I716207. <br> | ||
- | Digest pBca9145- | + | Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) <br> |
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br> | Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br> | ||
Product is pBca9145-I716208. <br> | Product is pBca9145-I716208. <br> |
Latest revision as of 18:56, 5 June 2007
[lox][term][lox]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
Digest pBca9145-I716205 (BamHI/AlwnI, 1723+553bp, Large)
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small)
Product is pBca9145-I716207.
Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large)
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small)
Product is pBca9145-I716208.