From 2007.igem.org

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Revision as of 21:03, 5 June 2007

NOT DONE YET...found an ALWNI site in Cre....

[rbs][ATG]
4 Different rbs variants (1106A, 1106B, 1128A, 1090)
2 Different Cre variants (212 and 213)

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Digest pBca1101-Bca1106A (BamHI/AlwnI/Xhol, 1588+850+544bp, Large)
Digest pBca9145-I716212 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716214.

Digest pBca1101-Bca1106B (BamHI/AlwnI/Xhol, 1588+850+544bp, Large)
Digest pBca9145-I716212 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716215.

Digest pBca1101-Bca1128A (BamHI/AlwnI/Xhol, 1606+850+544bp, Large)
Digest pBca9145-I716212 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716216.

Digest pBca9145-Bca1090 (BamHI/AlwnI/Xhol, 1538+553+9bp, Large)
Digest pBca9145-I716212 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716217.

Digest pBca1101-Bca1106A (BamHI/AlwnI/Xhol, 1588+850+544bp, Large)
Digest pBca9145-I716213 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716218.

Digest pBca1101-Bca1106B (BamHI/AlwnI/Xhol, 1588+850+544bp, Large)
Digest pBca9145-I716213(BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716219.

Digest pBca1101-Bca1128A (BamHI/AlwnI/Xhol, 1606+850+544bp, Large)
Digest pBca9145-I716213(BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716220.

Digest pBca9145-Bca1090 (BamHI/AlwnI/Xhol, 1538+553+9bp, Large)
Digest pBca9145-I716213(BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716221.