Arthur Yu Notebook
From 2007.igem.org
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[[Template:BerkiGEM2007_ArthurConstructionFiles | My Construction Files]]<br> | [[Template:BerkiGEM2007_ArthurConstructionFiles | My Construction Files]]<br> | ||
| - | [[Template:BerkiGEM2007_ArthurSequencingFiles | My Sequencing Files]]<br> | + | [[Template:BerkiGEM2007_ArthurSequencingFiles | My Sequencing Files]]<br><br> |
---- | ---- | ||
| + | ==6/5 coolbeans== | ||
| + | * Finalized oligos to order with Vai | ||
| + | * Learned about LB broth-ing and LB/Agar plating. Thanks, Austin and Sam :) | ||
| + | * Learned about the many composite part-making methods. Props 2 Chris | ||
| + | # prefix/suffix is weaksauce | ||
| + | # Use the AlwnI or BsaI or BglI, in conjuction with BglII or BamHI << (Did this today) | ||
| + | # DBBS | ||
| + | # 3 antibiotic; MIT endorses, used for BioBrick 1.0. Triple digest = bad | ||
| + | # 1-2-3 method << 'Our Goal' in a few weeks. should be leet. | ||
| + | * Planned and vicariously did the making of '''P_tet+RFP''' brick (see [[Vaibhavi_Umesh_Notebook | Vai Notebook]]) | ||
| + | <br> | ||
| + | ''Other Notes:'' All oligos are being ordered, w00t w00t. | ||
| + | [[User:Ayu|Ayu]] 18:36, 5 June 2007 (EDT) | ||
| + | <br> | ||
==6/4 Training Finishes, Real Stuff Starts== | ==6/4 Training Finishes, Real Stuff Starts== | ||
* Incubated some colonies | * Incubated some colonies | ||
Revision as of 22:36, 5 June 2007
My Construction Files
My Sequencing Files
6/5 coolbeans
- Finalized oligos to order with Vai
- Learned about LB broth-ing and LB/Agar plating. Thanks, Austin and Sam :)
- Learned about the many composite part-making methods. Props 2 Chris
- prefix/suffix is weaksauce
- Use the AlwnI or BsaI or BglI, in conjuction with BglII or BamHI << (Did this today)
- DBBS
- 3 antibiotic; MIT endorses, used for BioBrick 1.0. Triple digest = bad
- 1-2-3 method << 'Our Goal' in a few weeks. should be leet.
- Planned and vicariously did the making of P_tet+RFP brick (see Vai Notebook)
Other Notes: All oligos are being ordered, w00t w00t.
Ayu 18:36, 5 June 2007 (EDT)
6/4 Training Finishes, Real Stuff Starts
- Incubated some colonies
- Miniprep'd already-been-incubated colonies (2)
- Double digest of the 2 minipreps + parent plasmid
- Colony PCR'd the incubated E.coli
- Ran gel of the digest + PCR
- >>> PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder >>>
- No bands for PCR or parent. Confused? Other ones look great.
As for me: Wiki acc works now.
Designing oligos and will compare with Vai.
Ayu 18:19, 4 June 2007 (EDT)
to do
