Davidson Missouri W

From 2007.igem.org

Revision as of 14:43, 6 June 2007

===Davidson & Missouri Western Team Logos, iGEM2006===

Team Meeting Notes

Western Meeting Notes 051407 to Present [1]

Students

• Will DeLoache, Junior Biology Major, [2]

• Oyinade Adefuye, Senior Biology Major, [3]

• Jim Dickson , Junior Math and Economics Major, [4]

• Amber Shoecraft, ??? Major, [5]

• Andrew Martens, Senior Biology Major, [6]

• Michael Waters, Sophomore Biology Major, [7]

• Jordan Baumgardner, Junior Biology, Biochemistry/Molecular Biology Major, [8]

• Ryan Chilcoat, Junior Biology Major (Health Sciences), [9]

• Tom Crowley, Senior Biochemisty/Molecular Biology Major, [10]

• Lane H. Heard, Central High School graduate, [11]

• Nickolaus Morton, Junior Chemistry Major, [12]

• Michelle Ritter, Junior Mathematics Major, [13]

• Jessica Treece, Junior Biology Major (Health Sciences), [14]

• Matthew Unzicker, Senior Biochemistry/Molecular Biology Major, [15]

• Amanda Valencia, Senior Biochem/Molecular Biology Major, [16]

Faculty

• Malcolm Campbell [http://www.bio.davidson.edu/people/macampbell/macampbell.html], Professor, Department of Biology, [17]

• Karmella Haynes [http://www.bio.davidson.edu/people/kahaynes/kahaynes.html], Visiting Assistant Professor, Department of Biology, [18]

• Laurie Heyer [http://www.davidson.edu/math/heyer/], Associate Professor, Department of Mathematics, [19]

Shipping Address: Malcolm Campbell, Biology Dept. Davidson College, 209 Ridge Road, Davidson, NC 28036 [(704) 894-2692]

• Todd Eckdahl [http://staff.missouriwestern.edu/~eckdahl/], Professor, Department of Biology, [20]

• Jeff Poet [http://staff.missouriwestern.edu/~poet/], Assistant Professor, Department of Computer Science, Mathematics, and Physics, [21]

Shipping Address: Todd Eckdahl, Biology Department, Missouri Western State University, 4525 Downs Drive, Saint Joseph, MO, 64507 [(816) 271-5873]

Project Overview

Solving the Pancake Problem with an E. coli Computer Our goal is to genetically engineer a biological system that can compute solutions to a puzzle called the burnt pancake problem. The EHOP computer is a proof of concept for computing in vivo, with implications for future data storage devices and transgenic systems.

Resources / Citations

Davidson's Wet Lab Protocols

Cool site for Breakfast [http://www.cut-the-knot.org/SimpleGames/Flipper.shtml]

Karen Acker's paper describing GFP and TetA(c) with Hix insertions [http://www.bio.davidson.edu/Courses/Immunology/Students/spring2006/Acker/Acker_finalpaperGFP.doc]

Bruce Henschen's paper describing one-time flippable Hix sites [http://www.bio.davidson.edu/Courses/genomics/2006/henschen/Bruce_Finalpaper.doc]

Intro to Hamiltonian Path Problem and DNA [http://www.ams.org/featurecolumn/archive/dna-abc2.html]

Literature and Registry Research

Registry Search for Possible Promoters:

BBa_J24669 --- arabinose induced

BBa_R0082 --- Is upstream of the ompC porin gene

BBa_R0074 --- Penl regulated

BBa_I14017 --- P(Rhl)

BBa_I14018 --- P (Bla) --> amp resistance

BBa_J3902 --- Pr Fe (Pl + Pll rus operon)

BBa_R0077 --- CinR --> thought to have own terminator

BBa_R0078 --- CinR (no RBS)

BBa_R0062 --- luxR & HSL regulated -- luxpR

Possibly the use of Constitutive Promoter Family Members to strengthen other promoters.

Literature Search for Polycistronic Genes on Plasmids

Sol Operon http://www.jstage.jst.go.jp/article/bbb/71/1/58/_pdf

Transfer (tra) Operon http://www.pubmedcentral.nig.gov/picrender.fcgi?artid=1347297&blobtype=pdf

Oligopeptide Permease (opp) http://www.pubmedcentral.nig.gov/picrender.fcgi?artid=1087318&blobtype=pdf