Cloning in BioBrick vectors
From 2007.igem.org
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[[PHO80cds]] | [[PHO80cds]] | ||
| - | In the same time we have transformed plasmid pBS1A3[ | + | In the same time we have transformed plasmid pBS1A3[https://2007.igem.org/Biobrick_Vector_choice] in competent cells and after MIDI-prep we have loaded 1µl on the gel. |
We have digested in two differents ways our plasmid to be sure of it's identity! | We have digested in two differents ways our plasmid to be sure of it's identity! | ||
Then we have digested it with XbaI and SacII and we have loaded all digest product on gel to separate by gel extraction the insert from plasmid! | Then we have digested it with XbaI and SacII and we have loaded all digest product on gel to separate by gel extraction the insert from plasmid! | ||
Revision as of 14:42, 15 October 2007
We have amplified with PCR our promoters and Pho80 coding sequence and we have loaded 1µl on the gel to see if there were aspecific products or others. 1ORE-CYCtata
In the same time we have transformed plasmid pBS1A3[1] in competent cells and after MIDI-prep we have loaded 1µl on the gel. We have digested in two differents ways our plasmid to be sure of it's identity! Then we have digested it with XbaI and SacII and we have loaded all digest product on gel to separate by gel extraction the insert from plasmid!
