BerkiGEM2007-KristinConstructionFile1
From 2007.igem.org
(Difference between revisions)
Revision as of 21:08, 6 June 2007
Construction of hemA basic part from R. capsulatus PCR kd001/kd004 on hemA (639 bp, gp=A) PCR kd003/kd002 on hemA (618 bp, gp=B) -------------------------------------------------------- PCR kd001/kd002 on A+B (1237 bp, Bg1II/XhoI) Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) Product is pBca9145-I716151 -------------------------------------------------------- kd001 Forward Bg1II for hemA ccaaaAGATCTatggactacaatctcgcgctc kd002 Reverse Xho1 for hemA gctagCTCGAGttaGGATCCtcacgcacagcgcgcccagag kd003 Removing the EcoR1 site in hemA cgccgatGAGTTCggcgcgc kd004 Removing the EcoR1 site in hemA gcgcgccGAACTCatcggcg
