BerkiGEM2007-KristinConstructionFile3
From 2007.igem.org
(Difference between revisions)
Revision as of 21:11, 6 June 2007
Construction of hemC basic part PCR kd007/kd0010 on hemC (306 bp, gp=A) PCR kd009/kd008 on hemC (688 bp, gp=B) -------------------------------------------------------- PCR kd007/kd008 on A+B (994 bp, Bg1II/XhoI) Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) Product is pBca9145-I716154 -------------------------------------------------------- kd007 Forward Bg1II for hemC ccaaaAGATCTatgataatgacggtaacaagc kd008 Reverse Xho1 for hemC gctagCTCGAGttaGGATCCtcatgccggggcgtctccgttatag kd009 Forward remove the EcoR1 site in hemC gccggttGAGTTCccgcaag kd010 Reverse remove the EcoR1 site in hemC cttgcggGAACTCaaccggc
