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	CODING SEQUENCE'''		CODING SEQUENCE'''
-		+	ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATGAGGATCAAGGGATACCAAAA
-	1    ATGGAAAGCA CATCAGGAGA ACGTTCCGAA AATATACATG AGGATCAAGG	+	GTAATTCTGCCCGCTGATTTTAATAAATGCTCTAGAACTGACCTAGTGGTGCTCATATCA
-		+	CGAATGTTAGTATCGCTGATAGCAATCAATGAAAATTCAGCAACAAAGAAATCTGATGAC
-	51  GATACCAAAA GTAATTCTGC CCGCTGATTT TAATAAATGC TCTAGAACT	+	CAAATTACTTTAACACGATACCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTAT
-		+	TTCATACGACTGACAAAGTTTTCCTCTTTAGAACATTGTGTGCTTATGACATCACTCTAT
-	101  ACCTAGTGGT GCTCATATCA CGAATGTTAG TATCGCTGAT AGCAATCAAT	+	TATATCGATTTATTGCAAACTGTGTATCCTGATTTTACGCTTAATTCGTTGACTGCCCAT
-		+	AGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCTTATGTGATTCGTTCTCAACA
-	151  GAAAATTCAG CAACAAAGAA ATCTGATGAC CAAATTACTT TAACACGATA	+	AACGCCCATTATGCAAAAGTTGGAGGAGTACGATGTCACGAATTGAATATACTGGAGAAC
-		+	GATTTTTTAAAGAGAGTAAACTACAGAATCATTCCGCGGGATCATAACATTACGTTATGT
-	201  CCATTCTAAG ATTCCTCCAA ACATATCAAT CTTCAACTAT TTCATACGAC	+	AGTATAGAGCAAAAACAGAAAAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGAT
-		+	TTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGATATAATGTTCTAGATAAATAC
-	251  TGACAAAGTT TTCCTCTTTA GAACATTGTG TGCTTATGAC ATCACTCTAT	+	TATCGAAGAATAGTTCAGCTGGTGGGTTCCTTTAACGCTTCACCTGATAAGAGTAGAAAG
-		+	GTTGATTATGTTCTCCCGCCAAATATTGATATAGTGAGTGAAAGTGGCTCCCAAACTACT
-	301  TATATCGATT TATTGCAAAC TGTGTATCCT GATTTTACGC TTAATTCGTT	+	CAACTAAAGGGGTCGTCATCACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCA
-		+	AAGGACGCACATATCTATAACAAGCGATCAAAGCCAGATTAA
-	351  GACTGCCCAT AGGTTTTTAT TAACAGCCAC CACAGTCGCA ACAAAAGGCT	+
-		+
-	401  TATGTGATTC GTTCTCAACA AACGCCCATT ATGCAAAAGT TGGAGGAGTA	+
-		+
-	451  CGATGTCACG AATTGAATAT ACTGGAGAAC GATTTTTTAA AGAGAGTAAA	+
-		+
-	501  CTACAGAATC ATTCCGCGGG ATCATAACAT TACGTTATGT AGTATAGAGC	+
-		+
-	551  AAAAACAGAA AAAGTTTGTC ATAGATAAAA ACGCATTAGG GTCTCTCGAT	+
-		+
-	601  TTGGATTCTT ATTCTTACGT TAATCGTCCA AAAAGTGGAT ATAATGTTCT	+
-		+
-	651  AGATAAATAC TATCGAAGAA TAGTTCAGCT GGTGGGTTCC TTTAACGCTT	+
-		+
-	701  CACCTGATAA GAGTAGAAAG GTTGATTATG TTCTCCCGCC AAATATTGAT	+
-		+
-	751  ATAGTGAGTG AAAGTGGCTC CCAAACTACT CAACTAAAGG GGTCGTCATC	+
-		+
-	801  ACCCAATTCT CACTCTTCAC AAAAGCGATA TTCTGAGGCA AAGGACGCAC	+
-		+
-	851  ATATCTATAA CAAGCGATCA AAGCCAGATT AA	+
-		+
	XbaI cuts in 2 sites 91 and 648		XbaI cuts in 2 sites 91 and 648

---

Revision as of 10:45, 17 October 2007

Gene Pho80 CODING SEQUENCE

ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATGAGGATCAAGGGATACCAAAA GTAATTCTGCCCGCTGATTTTAATAAATGCTCTAGAACTGACCTAGTGGTGCTCATATCA CGAATGTTAGTATCGCTGATAGCAATCAATGAAAATTCAGCAACAAAGAAATCTGATGAC CAAATTACTTTAACACGATACCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTAT TTCATACGACTGACAAAGTTTTCCTCTTTAGAACATTGTGTGCTTATGACATCACTCTAT TATATCGATTTATTGCAAACTGTGTATCCTGATTTTACGCTTAATTCGTTGACTGCCCAT AGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCTTATGTGATTCGTTCTCAACA AACGCCCATTATGCAAAAGTTGGAGGAGTACGATGTCACGAATTGAATATACTGGAGAAC GATTTTTTAAAGAGAGTAAACTACAGAATCATTCCGCGGGATCATAACATTACGTTATGT AGTATAGAGCAAAAACAGAAAAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGAT TTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGATATAATGTTCTAGATAAATAC TATCGAAGAATAGTTCAGCTGGTGGGTTCCTTTAACGCTTCACCTGATAAGAGTAGAAAG GTTGATTATGTTCTCCCGCCAAATATTGATATAGTGAGTGAAAGTGGCTCCCAAACTACT CAACTAAAGGGGTCGTCATCACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCA AAGGACGCACATATCTATAACAAGCGATCAAAGCCAGATTAA

XbaI cuts in 2 sites 91 and 648

Pho80cds 882 bp e 293aa

XbaI restriction sequence TCTAGA

1)91 bp position 31-32aa SR S=TCT TCC TCA TCG

R=AGA AGG

2)648 bp position 216-217-218aa VLD V=TT GTC GTA GTG

L=TTA TTG CTT CTA CTC CTG

D=GAT GAC

5'3' Frame 1

Met E S T S G E R S E N I H E D Q G I P K V I L P A D F N K C S R T D L V V L I S R Met L V S L I A I N E N S A T K K S D D Q I T L T R Y H S K I P P N I S I F N Y F I R L T K F S S L E H C V L Met T S L Y Y I D L L Q T V Y P D F T L N S L T A H R F L L T A T T V A T K G L C D S F S T N A H Y A K V G G V R C H E L N I L E N D F L K R V N Y R I I P R D H N I T L C S I E Q K Q K K F V I D K N A L G S L D L D S Y S Y V N R P K S G Y N V L D K Y Y R R I V Q L V G S F N A S P D K S R K V D Y V L P P N I D I V S E S G S Q T T Q L K G S S S P N S H S S Q K R Y S E A K D A H I Y N K R S K P D Stop

Virtual Sequence: SEQUENCE 293 AA; 33227 MW; 72918CAF845AE74C CRC64.

    MESTSGERSE NIHEDQGIPK VILPADFNKC SRTDLVVLIS RMLVSLIAIN ENSATKKSDD
    QITLTRYHSK IPPNISIFNY FIRLTKFSSL EHCVLMTSLY YIDLLQTVYP DFTLNSLTAH
    RFLLTATTVA TKGLCDSFST NAHYAKVGGV RCHELNILEN DFLKRVNYRI IPRDHNITLC
    SIEQKQKKFV IDKNALGSLD LDSYSYVNRP KSGYNVLDKY YRRIVQLVGS FNASPDKSRK
    VDYVLPPNID IVSESGSQTT QLKGSSSPNS HSSQKRYSEA KDAHIYNKRS KPD

PHO80 Primer sequences:

t93c 5'-cgctgattttaataaatgctccagaactgacctagtggtgc-3'

t93c antisense 5'-gcaccactaggtcagttctggagcatttattaaaatcagcg-3'

t648c 5'-atcgtccaaaaagtggatataatgtcctagataaatactatcgaagaatag-3'

t648c antisense 5'-ctattcttcgatagtatttatctaggacattatatccactttttggacgat-3'