Paris/October 16
From 2007.igem.org
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Eismoustique (Talk | contribs) (→PCR rxn) |
Eismoustique (Talk | contribs) (→PCR rxn) |
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|pol= Phusion 0,5µL | |pol= Phusion 0,5µL | ||
|DNA= pY-2P-IntC 5µL | |DNA= pY-2P-IntC 5µL | ||
| - | |Size= | + | |Size=1Kb |
| - | |Success= | + | |Success=YES |
|Image= | |Image= | ||
|Band= | |Band= | ||
| Line 39: | Line 39: | ||
|DNA= pY-2P-IntC 1µL (dil. 1/10) | |DNA= pY-2P-IntC 1µL (dil. 1/10) | ||
|Size= | |Size= | ||
| - | |Success= | + | |Success= |
| + | |Image= | ||
| + | |Band= | ||
| + | |}} | ||
| + | |||
| + | {{Paris_PCR_0| Title = PrefixBB-mutDGAT-SufixBB | ||
| + | |Annealing= 0min | ||
| + | |Elongation= 2m | ||
| + | |Cycles= 33 | ||
| + | |Buffer= 5x 10µL (GC rich) | ||
| + | |MgCl2= 10µM 0µL (no extra) | ||
| + | |dNTP= 10µM 1µL | ||
| + | |n_oligoF= O29 | ||
| + | |v_oligoF= 10µM 4µL | ||
| + | |n_oligoR= O30 | ||
| + | |v_oligoR= 10µM 4µL | ||
| + | |water= qsp 50µL | ||
| + | |pol= Phusion 0,5µL | ||
| + | |DNA= P13 1µL (dil. 1/10) | ||
| + | |Size= | ||
| + | |Success= | ||
|Image= | |Image= | ||
|Band= | |Band= | ||
|}} | |}} | ||
Revision as of 12:08, 17 October 2007
PCR rxn
the following PCR reactions have been performed
| PCR : PrefixBB-frt1-CmR-frt2-SufixBB | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | 5x 10µL (GC rich) | Expected size | |||
| Annealing (°C) | MgCl2 10µM | 10µM 0µL (no extra) | 1Kb | |||
| 0min | dNTP 10µM | 10µM 1µL | Success | |||
| Time Elongation | Oligo F 10µM | O51 | 10µM 4µL | YES | ||
| 1m40' | Oligo R 10µM | O52 | 10µM 4µL | Image (click to enlarge) | ||
| Number cycles | Water | qsp 50µL | [[Image:|30px]] | |||
| 30 | Polymerase | Phusion 0,5µL | Band (0=ladder) | |||
| DNA | pY-2P-IntC 5µL | |||||
| PCR : PrefixBB-frt1-CmR-frt2-T1T2-SufixBB | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | 5x 10µL (GC rich) | Expected size | |||
| Annealing (°C) | MgCl2 10µM | 10µM 0µL (no extra) | ||||
| 0min | dNTP 10µM | 10µM 1µL | Success | |||
| Time Elongation | Oligo F 10µM | O51 | 10µM 4µL | |||
| 1m40' | Oligo R 10µM | O53 | 10µM 4µL | Image (click to enlarge) | ||
| Number cycles | Water | qsp 50µL | [[Image:|30px]] | |||
| 30 | Polymerase | Phusion 0,5µL | Band (0=ladder) | |||
| DNA | pY-2P-IntC 1µL (dil. 1/10) | |||||
| PCR : PrefixBB-mutDGAT-SufixBB | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | 5x 10µL (GC rich) | Expected size | |||
| Annealing (°C) | MgCl2 10µM | 10µM 0µL (no extra) | ||||
| 0min | dNTP 10µM | 10µM 1µL | Success | |||
| Time Elongation | Oligo F 10µM | O29 | 10µM 4µL | |||
| 2m | Oligo R 10µM | O30 | 10µM 4µL | Image (click to enlarge) | ||
| Number cycles | Water | qsp 50µL | [[Image:|30px]] | |||
| 33 | Polymerase | Phusion 0,5µL | Band (0=ladder) | |||
| DNA | P13 1µL (dil. 1/10) | |||||
