NYMU Taipei/Team schedule

From 2007.igem.org

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<h3>July 2007</h3>
 +
<p>
 +
<table style="text-align: left" width="50%" border="1">
 +
    <tbody>
 +
        <tr>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Sun.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Mon.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Tue.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Wed.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Thr.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Fri.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Sat.</big></td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">1</td>
 +
            <td align="center">2</td>
 +
            <td align="center">3</td>
 +
            <td align="center">4</td>
 +
            <td align="center">5</td>
 +
            <td align="center">[[2007/07/06|6]]</td>
 +
            <td align="center">7</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">8</td>
 +
            <td align="center">9</td>
 +
            <td align="center">10</td>
 +
            <td align="center">11</td>
 +
            <td align="center">12</td>
 +
            <td align="center">[[2007/07/13|13]]</td>
 +
            <td align="center">14</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">15</td>
 +
            <td align="center">[[2007/07/16|16]]</td>
 +
            <td align="center">17</td>
 +
            <td align="center">18</td>
 +
            <td align="center">19</td>
 +
            <td align="center">[[2007/07/20|20]]</td>
 +
            <td align="center">[[2007/07/21|21]]</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">22</td>
 +
            <td align="center">23</td>
 +
            <td align="center">[[2007/07/24|24]]</td>
 +
            <td align="center">25</td>
 +
            <td align="center">26</td>
 +
            <td align="center">27</td>
 +
            <td align="center">28</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">29</td>
 +
            <td align="center">30</td>
 +
            <td align="center">31</td>
 +
            <td align="center">&nbsp;</td>
 +
            <td align="center">&nbsp;</td>
 +
            <td align="center">&nbsp;</td>
 +
            <td align="center">&nbsp;</td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
</p>
 +
<p>&nbsp;</p>
 +
<h3>Aug 2007</h3>
 +
<p>
 +
<table style="text-align: left" width="50%" border="1">
 +
    <tbody>
 +
        <tr>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Sun.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Mon.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Tue.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Wed.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Thr.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Fri.</big></td>
 +
            <td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Sat.</big></td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">&nbsp;</td>
 +
            <td align="center">&nbsp;</td>
 +
            <td align="center">&nbsp;</td>
 +
            <td align="center">1</td>
 +
            <td align="center">2</td>
 +
            <td align="center">3</td>
 +
            <td align="center">4</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">5</td>
 +
            <td align="center">[[2007/08/06|6]]</td>
 +
            <td align="center">7</td>
 +
            <td align="center">8</td>
 +
            <td align="center">9</td>
 +
            <td align="center">10</td>
 +
            <td align="center">11</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">12</td>
 +
            <td align="center">13</td>
 +
            <td align="center">14</td>
 +
            <td align="center">15</td>
 +
            <td align="center">16</td>
 +
            <td align="center">17</td>
 +
            <td align="center">18</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">19</td>
 +
            <td align="center">20</td>
 +
            <td align="center">21</td>
 +
            <td align="center">22</td>
 +
            <td align="center">23</td>
 +
            <td align="center">24</td>
 +
            <td align="center">25</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">26</td>
 +
            <td align="center">27</td>
 +
            <td align="center">28</td>
 +
            <td align="center">29</td>
 +
            <td align="center">30</td>
 +
            <td align="center">31</td>
 +
            <td align="center">&nbsp;</td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
</p>
 +
<p>&nbsp;</p>
 +
<h3>System 1 assembly</h3>
 +
<div align="center">&nbsp;</div>
 +
<p>
 +
<table border="1">
 +
    <tbody>
 +
        <tr>
 +
            <td align="center"><strong>Run</strong></td>
 +
            <td align="center"><strong>Date and Time<br />
 +
            </strong></td>
 +
            <td align="center"><strong>Objective</strong></td>
 +
            <td align="center"><strong>Attendance</strong></td>
 +
        </tr>
 +
        <tr>
 +
            <td style="background-color: rgb(255,255,255)" align="center">1</td>
 +
            <td style="background-color: rgb(255,255,255)">8 AM Sat, 9/1, 07</td>
 +
            <td style="background-color: rgb(255,255,255)">
 +
            <ul>
 +
                <li>(RBS + cinR) + (RBS + HSL) = cinR + HSL</li>
 +
                <li>RBS + cinR ver. 2</li>
 +
                <li>plate culture for (cinR + HSL) and (RBS + CinR) ver.2</li>
 +
            </ul>
 +
            </td>
 +
            <td style="background-color: rgb(255,255,255)" align="center">茹, 翔</td>
 +
        </tr>
 +
        <tr>
 +
            <td style="background-color: rgb(255,255,255)" align="center">2</td>
 +
            <td style="background-color: rgb(255,255,255)">8 AM Sun, 9/2, 07</td>
 +
            <td style="background-color: rgb(255,255,255)">
 +
            <ul>
 +
                <li>Liquid culture for (cinR + HSL) and (RBS + CinR) ver.2</li>
 +
                <li>[[classify parts in box]]</li>
 +
            </ul>
 +
            </td>
 +
            <td style="background-color: rgb(255,255,255)" align="center">雄, 威, 茹, 駿</td>
 +
        </tr>
 +
        <tr>
 +
            <td style="background-color: rgb(255,255,255)" align="center">3</td>
 +
            <td style="background-color: rgb(255,255,255)">8 AM Mon, 9/3, 07</td>
 +
            <td style="background-color: rgb(255,255,255)">
 +
            <ul>
 +
                <li>plasmid extraction for (cinR + HSL) and (RBS + CinR) ver.2</li>
 +
                <li>pOmpC + (RBS + INS_A)</li>
 +
                <li>(RBS + cinR) + (RBS + HSL) + D-term</li>
 +
            </ul>
 +
            </td>
 +
            <td style="background-color: rgb(255,255,255)" align="center">宥, 君, 威</td>
 +
        </tr>
 +
        <tr>
 +
            <td style="background-color: rgb(255,255,255)" align="center">4</td>
 +
            <td style="background-color: rgb(255,255,255)">8 AM Tue, 9/4, 07</td>
 +
            <td style="background-color: rgb(255,255,255)">
 +
            <ul>
 +
                <li>plate culture for ...</li>
 +
                <li>Measure the concentration of all excised parts
 +
                <ul>
 +
                    <li>(cinR + HSL)</li>
 +
                    <li>(RBS + CinR) ver.2</li>
 +
                </ul>
 +
                </li>
 +
                <li>BsaBI digestion of cinR</li>
 +
            </ul>
 +
            </td>
 +
            <td style="background-color: rgb(255,255,255)" align="center">宥, 君, 威</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">5</td>
 +
            <td>8 AM Wed, 9/5, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>(RBS + INS_A), PCR #1</li>
 +
                <li>(RBS + INS_B), PCR #1</li>
 +
                <li>pOmpC + (RBS + INS_A), not check yet</li>
 +
                <li>pOmpC + (RBS + INS_B), not check yet</li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">威, 茹</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">6</td>
 +
            <td>8 AM Thr, 9/6, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>N/A</li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">威</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">7</td>
 +
            <td>8 AM Fri, 9/7, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>N/A</li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">儒, 威, 茹</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">8</td>
 +
            <td>8 AM Sat, 9/8, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>(RBS+INS_A), PCR #2</li>
 +
                <li>(RBS+INS_B), PCR #2</li>
 +
                <li>GEL check (5 tubes for each gene)
 +
                <ul>
 +
                    <li>INS_A has two tubes A1 (0.08g), A3 (0.15g)</li>
 +
                    <li>INS_B has five tubes B1 (0.12g), B2 (0.12g), B3 (0.16g), B4 (0.11g), B5 (0.17g)</li>
 +
                </ul>
 +
                </li>
 +
                <li>ligation for INS_A and pOmpC</li>
 +
                <li>plate culture for (pOmpC+INS_A and pOmpC+INS_B), transformation <font color="#ff0000">failed</font>
 +
                <ul>
 +
                    <li>forget to add vector</li>
 +
                </ul>
 +
                </li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">倫, 茹</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">9</td>
 +
            <td>8 AM Sun, 9/9, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>plate culture <font color="#ff0000">failed</font> (possible due to absent of vector, checked by text paper record)</li>
 +
                <li>re-ligate INS_A insert into vector with pOmpC</li>
 +
                <li>re-check the concentration of insert INS_A by <a href="http://rsb.info.nih.gov/ij/">imageJ</a></li>
 +
                <li>plate culture again for pOmpC+INS_A
 +
                <ul>
 +
                    <li>1uL insert + 1uL vector</li>
 +
                </ul>
 +
                </li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">倫, 翔</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">10</td>
 +
            <td>4 PM Mon, 9/10, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>plate culture is still <font color="#ff0000">failed</font></li>
 +
                <li>re-build pOmpC + INS_A in two different insert concentration
 +
                <ul>
 +
                    <li>2uL insert + 1uL vector</li>
 +
                    <li>3uL insert + 1uL vector</li>
 +
                </ul>
 +
                </li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">倫, 翔, 銘</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">11</td>
 +
            <td>6 PM Thu, 9/11, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>re-measure the concentration of insert by biophotometer
 +
                <ul>
 +
                    <li>dsDNA use A260 OD to estimate, 1 A260 OD = 50 mg/mL = 0.05 ug/uL</li>
 +
                    <li>OD ranged from 0.1 - 2.0 is located in linear region</li>
 +
                    <li>OD close to zero is not reliable</li>
 +
                </ul>
 +
                </li>
 +
                <li>test for D7 and D8 (9/8 PCR #2) in box #1</li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">銘</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">12</td>
 +
            <td>6 PM Wed, 9/12, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>check (9/5 PCR) by GEL separation
 +
                <ul>
 +
                    <li>concentration of INS_A is still too low</li>
 +
                </ul>
 +
                </li>
 +
                <li>RE-examine the condition of PCR in different annealing temperature
 +
                <ul>
 +
                    <li>PCR INS_A in 49.8, 52.3, 55.5, 58.7 and 60.9 degree</li>
 +
                </ul>
 +
                </li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">銘</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">13</td>
 +
            <td>6 PM Thur, 9/13, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>check (9/12 PCR) by GEL separation
 +
                <ul>
 +
                    <li>band seems O.K.</li>
 +
                    <li>best annealing temperature is around 55.5</li>
 +
                </ul>
 +
                </li>
 +
                <li>biophotometer concentration test
 +
                <ul>
 +
                    <li>9/5 and 9/12 PCR seem O.K.</li>
 +
                    <li>9/8 PCR seem problemistic due to A260/A280 more than 10</li>
 +
                </ul>
 +
                </li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">銘</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">14</td>
 +
            <td>6 PM Sun, 9/16, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>digest CinR+HSL (ES) and D-term (EX)and GEL separation check
 +
                <ul>
 +
                    <li>tube #1 and #2 of CinR+HSL plasmid extraction is not correct after GEL check</li>
 +
                    <li>first digestion of D-term (EcoRI) is not very sucessful
 +
                    <ul>
 +
                        <li>thus pett re-digest D-term with double enzyme amount</li>
 +
                    </ul>
 +
                    </li>
 +
                </ul>
 +
                </li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">宥, 君, 翔</td>
 +
        </tr>
 +
        <tr>
 +
            <td align="center">PCR</td>
 +
            <td>Thur, 9/27, 07</td>
 +
            <td>
 +
            <ul>
 +
                <li>PCR of TAT_INS_A, TAT_INS_B, OmpRBS, TAT_IDE</li>
 +
            </ul>
 +
            </td>
 +
            <td align="center">威</td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
</p>
 +
<p>&nbsp;</p>
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[https://2007.igem.org/NYMU_Taipei/Welcome Back]

Revision as of 14:23, 18 October 2007

July 2007

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Aug 2007

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System 1 assembly

 

<tbody> </tbody>
Run Date and Time
 Objective Attendance
1 8 AM Sat, 9/1, 07
  • (RBS + cinR) + (RBS + HSL) = cinR + HSL
  • RBS + cinR ver. 2
  • plate culture for (cinR + HSL) and (RBS + CinR) ver.2
 茹, 翔
2 8 AM Sun, 9/2, 07 雄, 威, 茹, 駿
3 8 AM Mon, 9/3, 07
  • plasmid extraction for (cinR + HSL) and (RBS + CinR) ver.2
  • pOmpC + (RBS + INS_A)
  • (RBS + cinR) + (RBS + HSL) + D-term
 宥, 君, 威
4 8 AM Tue, 9/4, 07
  • plate culture for ...
  • Measure the concentration of all excised parts
    • (cinR + HSL)
    • (RBS + CinR) ver.2
  • BsaBI digestion of cinR
 宥, 君, 威
5 8 AM Wed, 9/5, 07
  • (RBS + INS_A), PCR #1
  • (RBS + INS_B), PCR #1
  • pOmpC + (RBS + INS_A), not check yet
  • pOmpC + (RBS + INS_B), not check yet
 威, 茹
6 8 AM Thr, 9/6, 07
  • N/A
 威
7 8 AM Fri, 9/7, 07
  • N/A
 儒, 威, 茹
8 8 AM Sat, 9/8, 07
  • (RBS+INS_A), PCR #2
  • (RBS+INS_B), PCR #2
  • GEL check (5 tubes for each gene)
    • INS_A has two tubes A1 (0.08g), A3 (0.15g)
    • INS_B has five tubes B1 (0.12g), B2 (0.12g), B3 (0.16g), B4 (0.11g), B5 (0.17g)
  • ligation for INS_A and pOmpC
  • plate culture for (pOmpC+INS_A and pOmpC+INS_B), transformation failed
    • forget to add vector
 倫, 茹
9 8 AM Sun, 9/9, 07
  • plate culture failed (possible due to absent of vector, checked by text paper record)
  • re-ligate INS_A insert into vector with pOmpC
  • re-check the concentration of insert INS_A by <a href="http://rsb.info.nih.gov/ij/">imageJ</a>
  • plate culture again for pOmpC+INS_A
    • 1uL insert + 1uL vector
 倫, 翔
10 4 PM Mon, 9/10, 07
  • plate culture is still failed
  • re-build pOmpC + INS_A in two different insert concentration
    • 2uL insert + 1uL vector
    • 3uL insert + 1uL vector
 倫, 翔, 銘
11 6 PM Thu, 9/11, 07
  • re-measure the concentration of insert by biophotometer
    • dsDNA use A260 OD to estimate, 1 A260 OD = 50 mg/mL = 0.05 ug/uL
    • OD ranged from 0.1 - 2.0 is located in linear region
    • OD close to zero is not reliable
  • test for D7 and D8 (9/8 PCR #2) in box #1
 銘
12 6 PM Wed, 9/12, 07
  • check (9/5 PCR) by GEL separation
    • concentration of INS_A is still too low
  • RE-examine the condition of PCR in different annealing temperature
    • PCR INS_A in 49.8, 52.3, 55.5, 58.7 and 60.9 degree
 銘
13 6 PM Thur, 9/13, 07
  • check (9/12 PCR) by GEL separation
    • band seems O.K.
    • best annealing temperature is around 55.5
  • biophotometer concentration test
    • 9/5 and 9/12 PCR seem O.K.
    • 9/8 PCR seem problemistic due to A260/A280 more than 10
 銘
14 6 PM Sun, 9/16, 07
  • digest CinR+HSL (ES) and D-term (EX)and GEL separation check
    • tube #1 and #2 of CinR+HSL plasmid extraction is not correct after GEL check
    • first digestion of D-term (EcoRI) is not very sucessful
      • thus pett re-digest D-term with double enzyme amount
 宥, 君, 翔
PCR Thur, 9/27, 07
  • PCR of TAT_INS_A, TAT_INS_B, OmpRBS, TAT_IDE
 威

 

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