Boston UniversityPlasmidChoice
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== Plasmid Choice== | == Plasmid Choice== | ||
| - | Our plan is to replicate our engineered plasmid in E. coli and then transfer the plasmid to S. oneidensis through conjugation. In order to allow for conjugation, an oriT site must be present, and we have chosen to use the plasmid PJQ200 because it has this site. The normal form of PJQ200 contains a sacB | + | Our plan is to replicate our engineered plasmid in E. coli and then transfer the plasmid to S. oneidensis through conjugation. In order to allow for conjugation, an oriT site must be present, and we have chosen to use the plasmid PJQ200 because it has this site. The normal form of PJQ200 contains a sacB gene, which in the presence of sucrose is lethal in non-enterobacteria (and S. oneidensis is not an enterobacterium). In order to not harm S. oneidensis after the conjugation, the plasmid will be engineered beforehand so the sacB gene is not present. |
This is a [https://www.atcc.org/common/images/vectors/gifs/77482.gif map] of PJQ200, not engineered. | This is a [https://www.atcc.org/common/images/vectors/gifs/77482.gif map] of PJQ200, not engineered. | ||
Revision as of 15:37, 8 June 2007
Plasmid Choice
Our plan is to replicate our engineered plasmid in E. coli and then transfer the plasmid to S. oneidensis through conjugation. In order to allow for conjugation, an oriT site must be present, and we have chosen to use the plasmid PJQ200 because it has this site. The normal form of PJQ200 contains a sacB gene, which in the presence of sucrose is lethal in non-enterobacteria (and S. oneidensis is not an enterobacterium). In order to not harm S. oneidensis after the conjugation, the plasmid will be engineered beforehand so the sacB gene is not present.
This is a map of PJQ200, not engineered.
