BerkiGEM2007-VaibhaviConstructionFile2
From 2007.igem.org
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Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the SodC open reading frame from Escherichia coli K-12 MG1655 | Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the SodC open reading frame from Escherichia coli K-12 MG1655 | ||
<pre> | <pre> | ||
| - | PCR VU01F.sodC/VU02R.sodC on SodC | + | PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI) |
Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) | Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) | ||
Product is pBca9145-VU1 | Product is pBca9145-VU1 | ||
Revision as of 22:31, 8 June 2007
Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the SodC open reading frame from Escherichia coli K-12 MG1655
PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI) Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) Product is pBca9145-VU1 ------------------------------------- VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc
