From 2007.igem.org

Revision as of 19:58, 21 October 2007

An actual demonstration is decided to show the extensible ability of our method.

This demo system

• is designed as simple as possible, without no "cool" logic function;
• includes all the three logic gates, which form into a three-level logic circuit;
• shows that wires can cross and branch off (without interference);
• is loaded on two plasmids, [http://partsregistry.org/Part:BBa_I732998 pSB1A3-I732998] and [http://partsregistry.org/Part:BBa_I732999 pSB1A3-I732999] ,and transformed into Top10 strain in practice;

• accepts aTc and AHL signals as inputs;
• produces RFP and GFP signals as outputs;
• can be "resetted" by IPTG signal (i.e. all outputs lighten when IPTG added in);
• is expected to output the results as the truth table shown in Figure 2.

Logic Abstract of the Demo

Figure 1 The logic diagram of the demo system.

Figure 2 The truth table of the demo system.

In this truth table we can see that IPTG might result in all outputs lighten whether aTc or AHL exsit. (It just like the "8888..." on the screen when you reset your calculator or some other digital equipments.)

Actual System

Figure 3 The signal pathway of the demo system.

The left three parts are loaded on [http://partsregistry.org/Part:BBa_I732998 pSB1A3-I732998], and the rest two parts are loaded on [http://partsregistry.org/Part:BBa_I732999 pSB1A3-I732999]. We've built up this system in one kind of TOP10 E.coli, but it seems that it cannot grow stably when aTc exist.(We have to use aTc at a high concentration since the high expression of LuxR.) I may not show the final results here before Oct. 26, but I'm trying my best to filter out a monoclonal strain for that. You will get the results in the coming presentation...