Imperial/Infector Detector/Design
From 2007.igem.org
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__NOTOC__ | __NOTOC__ | ||
| - | + | = Infector Detector: Design = | |
| + | |||
| + | == Design Overview == | ||
| + | <center>[[Image:InfectorDetectorDesignWiki.png]] </center> | ||
| + | <br> | ||
| + | |||
| + | {|border="1" | ||
| + | |- | ||
| + | |width="100px"|'''Property''' | ||
| + | |width="300px"|'''Value''' | ||
| + | |width="300px"|'''Design Solution''' | ||
| + | |width="100px"|'''System Level''' | ||
| + | |- style="background:#eeffee" | ||
| + | |Health Regulations | ||
| + | |System Must not be living replicating bacteria | ||
| + | |Use a Cell Free System e.g. Promega's S30 Cell Extract | ||
| + | |Chassis | ||
| + | |- style="background:#eeffee" | ||
| + | |Lifespan | ||
| + | |System must have a shelf life of 7 days | ||
| + | |Protease Inhibitor of Cell Extract Should ensure degradation of Visual Reporter is Minimal<br> Proper Packaging should ensure that evaporation of Cell Free system is so low that <br> system can surive for 7 days | ||
| + | |Chassis | ||
| + | |- style="background:#eeeeff" | ||
| + | |Inputs | ||
| + | |System must be sensitive to AHL concentration between 5-50nM | ||
| + | |Sensitive AHL receiver to detect low AHL levels | ||
| + | |Construct | ||
| + | |||
| + | |||
| + | |- style="background:#eeeeff" | ||
| + | | | ||
| + | | | ||
| + | |||
| + | |||
| + | |} | ||
| + | |||
== Gene Construct == | == Gene Construct == | ||
Revision as of 21:36, 21 October 2007
Infector Detector: Design
Design Overview
| Property | Value | Design Solution | System Level |
| Health Regulations | System Must not be living replicating bacteria | Use a Cell Free System e.g. Promega's S30 Cell Extract | Chassis |
| Lifespan | System must have a shelf life of 7 days | Protease Inhibitor of Cell Extract Should ensure degradation of Visual Reporter is Minimal Proper Packaging should ensure that evaporation of Cell Free system is so low that system can surive for 7 days | Chassis |
| Inputs | System must be sensitive to AHL concentration between 5-50nM | Sensitive AHL receiver to detect low AHL levels | Construct
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