Tokyo/Assay
From 2007.igem.org
(Difference between revisions)
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<br>- to practice and experience the assays with AHL and IPTG | <br>- to practice and experience the assays with AHL and IPTG | ||
<br>- to check if and how our materials actually worked. | <br>- to check if and how our materials actually worked. | ||
+ | |||
+ | == [[Tokyo/AHL preparation |AHL preparation]] == | ||
+ | == [[Tokyo/Experimental precision and accurecy|Experimental precision and accurecy]] == | ||
= [[Tokyo/Assays for hybrid promoter|Assays for hybrid promoter]] = | = [[Tokyo/Assays for hybrid promoter|Assays for hybrid promoter]] = |
Revision as of 00:21, 22 October 2007
Contents |
Hill function fitting
新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める.
ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
導出に関してはfor more detail.
Preliminary assays
Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.
AHL preparation
Experimental precision and accurecy
Assays for hybrid promoter
These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.