Week 17

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
::'''10/22/07'''
::'''10/22/07'''
*Ligation:
*Ligation:
-
::-pLac-LacY-GFP on pSB4A3;
+
::-I763032 on pSB4A3;
*Transformation:
*Transformation:
-
::-pLac-LacY-GFP on pSB4A3;
+
::-I763032 on pSB4A3;
*We inoculate:
*We inoculate:
-
::-pLac-GFP-pTet-LacI (on high copy plasmid);
+
::-I763040 (on high copy plasmid);
-
::-pLac-cI-GFP (on low copy plasmid), 3 different inocules using 3 different colonies;
+
::-I763031 (on low copy plasmid), 3 different inocules using 3 different colonies;
-
::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).
+
::-I763029 on pSB4A3 (high copy plasmid).
-
::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).
+
::-I763029 on pSB4A3 (low copy plasmid).

Revision as of 08:55, 23 October 2007

10/22/07
  • Ligation:
-I763032 on pSB4A3;
  • Transformation:
-I763032 on pSB4A3;
  • We inoculate:
-I763040 (on high copy plasmid);
-I763031 (on low copy plasmid), 3 different inocules using 3 different colonies;
-I763029 on pSB4A3 (high copy plasmid).
-I763029 on pSB4A3 (low copy plasmid).



10/23/07



10/24/07



10/25/07



10/26/07



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