Freiburg07/report light sensor
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Revision as of 13:46, 23 October 2007
Contents |
Towards optical signal processing in E. coli
iGEM team Freiburg
Introduction
Phytochrome A (PhyA) is a member of the phytochrome family of red/far-red photo-
receptors. These photoreceptors are involved in a wide range of developmental
responses in plants. Fhy1 is a protein which binds PhyA under light conditions,
enable us to use this protein-protein interaction. In plants Fhy1 is responsable
for nuclear accumulation of PhyA in case of light exposure.
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Materials and Methods
Cloning of constructs:
PhyA Insert was gained by PCR from plasmid D153ah-phyA using 5´ (5´-TATCGACGAA TTCGCGGCCG CTTCTAGAAA GAGGAGAAAT
TAACTATGTC AGGCTCTAGG CCGAC-3´) and 3´ (5´- AACGATCACT AGTTCCGCCG TGATGGTGAT GGTGGCCTCC GTTATCGAGT TCCACCTCCT TA-3´) primers.
Fhy1 Insert1 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCGATTACA TCTATGGGAC TCAGA-3´) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.
Fhy1 Insert2 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCAGTAGTA ATGCTGCTAA GTTTGT-3`) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.
