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Celluar Lead Sensor Lead poisoning is a public health concern - there is lead in soil, paint, water, and dust. Lead Poisoning is often caused by ingesting contaminated drinking water, or soil. It can cause neurological and gastrointestinal disorders, especially among children. The legal limit of lead in drinking water is 15 parts per billion. Current ways of testing for lead either require expensive chemical lab analysis or involve inaccurate home kits. A lead detector, based on E. Col, is cheap, sensitive, quick, and specific Our system involves a Lead detecting promoter, an amplifier, and an output of GFP. We're also working on a system to remove false positives.			Tristable Switch The Tri-Stable Toggle Switch represents a continuation on the theme of the Toggle Switch begun by Gardner, et al 2000. The switch can be turned to one of three different outputs and will remain stable until a new input is added to the system. Applications for this system have been suggested such as cellular memory. Our approach to solving the switch is based on quantitative measurements in pioneering effort to establish a standardized means of designing and debugging biological systems. the Gardner paper presented a mathematical model of the system to illustrate important parameters. This project provides proof of concept for an "n"-stable Toggle Switch. The purpose of the Tri-stable Toggle Switch is to produce three distinct, continuous, and stable outputs in response to three distinct inputs. These three inputs are three separate chemicals which will each induce one state of the switch. In order to achieve this goal, we are constructing three constructs, each of which consists of a repressible, constitutively-on promoter attached to two repressors. Specifically, our three constructs are pBAD->LacI->TetR, pLacI->AraC->TetR, and pTet->AraC->LacI, where AraC represses pBAD, LacI represses pLac and TetR represses pTet. Each of the three repressors are inactivated by one of three chemicals, the three inducer chemicals mentioned earlier. These three(arabinose, IPTG (Isopropyl β-D-1-thiogalactopyranoside) and Tetracycline, respectively), cause conformational changes in their respective repressor proteins which leads to gene expression. For example, in the presence of arabinose, AraC cannot repress pBAD so LacI and TetR are produced which in turn repress pTet and pLac.

News Updates

08.28.07

Nanodrop donates ND-1000 Spectrophotometer and ND-3300 Fluorospectrophotometer to Brown iGEM

08.22.07

Brown iGEM Team presents its August Update to faculty and friends

08.20.07

Nanodrop it like it's hot! (YouTube Link)

08.08.07

We'll be hosting several iGEM Teams here at Brown University on August 18th for the iGEM 2007 Meetup. Teams will present their progress to each other and discuss the future of Synthetic Biology.

06.07.07

$25,000 grant from Pfizer for equipment purchases

06.01.07

Labnet sponsors Brown iGEM with vortex mixers, a microcentrifuge, pipettes, and autopipettes.

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Brown

From 2007.igem.org

Revision as of 00:12, 24 October 2007

@@ Line 95: / Line 95: @@
 <span style="font-size:18pt">Tristable Switch</span>
 <p  style="font-size: 13pt">
+The Tri-Stable Toggle Switch represents a continuation on the theme of the Toggle Switch begun by Gardner, et al 2000.  The switch can be turned to one of three different outputs and will remain stable until a new input is added to the system.  Applications for this system have been suggested such as cellular memory.  Our approach to solving the switch is based on quantitative measurements in pioneering effort to establish a standardized means of designing and debugging biological systems.  the Gardner paper presented a mathematical model of the system to illustrate important parameters.
+This project provides proof of concept for an "n"-stable Toggle Switch.
 The purpose of the Tri-stable Toggle Switch is to produce three distinct, continuous, and stable outputs in response to three distinct inputs. These three inputs are three separate chemicals which will each induce one state of the switch.