Tokyo/Preliminary assays

From 2007.igem.org

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[[Image:AHL Assay2.JPG|thumb|200px| '''Fig.1: '''Exogenously added AHL]]
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Preliminaly assays were done  
Preliminaly assays were done  
<br>- to practice and experience the assays with AHL and IPTG
<br>- to practice and experience the assays with AHL and IPTG

Revision as of 04:59, 24 October 2007

Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

Contents

AHL preparation

Experimental precision and accurecy

AHL assay practice

Lux-lac hybrid promoter activation check

OD correction

Wash

New AHL check

Hybrid promoter activation & repression check