Freiburg07/Ligation
From 2007.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
'''Ligation''' | '''Ligation''' | ||
+ | total volume: 10 µl | ||
+ | |||
+ | 2 µl vector DNA | ||
+ | 6 µl insert DNA | ||
1 µl ligase | 1 µl ligase | ||
- | 1 µl ligase buffer | + | 1 µl ligase buffer (1:10) |
- | + |
Revision as of 12:56, 24 October 2007
Ligation
total volume: 10 µl
2 µl vector DNA 6 µl insert DNA 1 µl ligase 1 µl ligase buffer (1:10)