Freiburg07/Transformation
From 2007.igem.org
(Difference between revisions)
Revision as of 14:44, 24 October 2007
Transformation of frozen competent cells
1. Thaw frozen cells on ice, 10-15 minutes.
2. Add 80 µl cells to the 20 µl ligation reaction.
3. Incubate on ice for 5 minutes.
4. Heat shock cells for 10 minutes at 37 °C.
5. Bring up in 1 mL LB and shake gently for 1 hour at 37 °C.
