Freiburg07/Transformation
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| - | Transformation of frozen competent cells | + | '''Transformation of frozen competent cells''' |
| - | 1. Thaw frozen cells | + | 1. Thaw frozen cells.<BR> |
| - | 2. Add | + | 2. Add 10 µl ligation and shake gent<BR> |
| - | 3. Incubate on ice for 5 minutes. | + | 3. Incubate on ice for 5-10 minutes.<br> |
4. Heat shock cells for 10 minutes at 37 °C. | 4. Heat shock cells for 10 minutes at 37 °C. | ||
5. Bring up in 1 mL LB and shake gently for 1 hour at 37 °C. | 5. Bring up in 1 mL LB and shake gently for 1 hour at 37 °C. | ||
Revision as of 14:52, 24 October 2007
Transformation of frozen competent cells
1. Thaw frozen cells.
2. Add 10 µl ligation and shake gent
3. Incubate on ice for 5-10 minutes.
4. Heat shock cells for 10 minutes at 37 °C.
5. Bring up in 1 mL LB and shake gently for 1 hour at 37 °C.
