ETHZ/primer pcr killspe up
From 2007.igem.org
(Difference between revisions)
Revision as of 21:31, 24 October 2007
We made the site directed mutagenesis with help of PCR. The sequence for the Primer to "kill" the SpeI site in the origin of replication is:
CAA CTC AAA GGA AAA GGA CTA ATA ATT ATC ATT GAC TAG
