Brown
From 2007.igem.org
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Wouldn't you like to have a stable signal in the continously fluctuating environment in cells? And if, say, your system doesn't work the very first try, wouldn't you like to have a standardized means to go about debugging it? | Wouldn't you like to have a stable signal in the continously fluctuating environment in cells? And if, say, your system doesn't work the very first try, wouldn't you like to have a standardized means to go about debugging it? | ||
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The Tri-Stable Toggle Switch represents a continuation on the theme of the Toggle Switch begun by Gardner, et al 2000. The switch produces three distinct and stable outputs in response to three distinct inputs. Our approach to designing the switch is based on quantitative measurements in a pioneering effort to establish a standardized means of designing and debugging biological systems. | The Tri-Stable Toggle Switch represents a continuation on the theme of the Toggle Switch begun by Gardner, et al 2000. The switch produces three distinct and stable outputs in response to three distinct inputs. Our approach to designing the switch is based on quantitative measurements in a pioneering effort to establish a standardized means of designing and debugging biological systems. | ||
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The purpose of the Tri-stable Toggle Switch is to produce three distinct, continuous, and stable outputs in response to three distinct inputs. These three inputs are three separate chemicals which will each induce one state of the switch.--> | The purpose of the Tri-stable Toggle Switch is to produce three distinct, continuous, and stable outputs in response to three distinct inputs. These three inputs are three separate chemicals which will each induce one state of the switch.--> | ||
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<!--In order to achieve this goal, we are constructing three constructs, each of which consists of a repressible, constitutively-on promoter attached to two repressors. Specifically, our three constructs are pBAD->LacI->TetR, pLacI->AraC->TetR, and pTet->AraC->LacI, where AraC represses pBAD, LacI represses pLac and TetR represses pTet. Each of the three repressors are inactivated by one of three chemicals, the three inducer chemicals mentioned earlier. These three(arabinose, IPTG (Isopropyl β-D-1-thiogalactopyranoside) and Tetracycline, respectively), cause conformational changes in their respective repressor proteins which leads to gene expression. For example, in the presence of arabinose, AraC cannot repress pBAD so LacI and TetR are produced which in turn repress pTet and pLac.--> | <!--In order to achieve this goal, we are constructing three constructs, each of which consists of a repressible, constitutively-on promoter attached to two repressors. Specifically, our three constructs are pBAD->LacI->TetR, pLacI->AraC->TetR, and pTet->AraC->LacI, where AraC represses pBAD, LacI represses pLac and TetR represses pTet. Each of the three repressors are inactivated by one of three chemicals, the three inducer chemicals mentioned earlier. These three(arabinose, IPTG (Isopropyl β-D-1-thiogalactopyranoside) and Tetracycline, respectively), cause conformational changes in their respective repressor proteins which leads to gene expression. For example, in the presence of arabinose, AraC cannot repress pBAD so LacI and TetR are produced which in turn repress pTet and pLac.--> | ||
Revision as of 00:29, 25 October 2007
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