Tianjin/DIODE

From 2007.igem.org

(Difference between revisions)

Revision as of 12:20, 25 October 2007

Design

The diode, one of the most significant parts in the electric integrate circuit and electronic equipment, functions as allowing the current get through when the diode is positive-biased, and cut off while it is negative-biased. When the negative voltage increases to a threshold, the diode tends to be negatively broken down. In this project, the molecular signal AHL is considered as the current of electric circuit.Three types of cells: generator cell、amplifier cells and block cells are immobilized into small beads with a diameter of 0.5 millimeter, then different beads are settled into particular parts of our specially designed equipment sequentially and respectively.
The liquid culture flow through the generator cells and carry the AHL signal into the amplifier cells which are activated to make more AHL signals.
Then the concentration of AHL accumulates to such a level that block cells could not eliminate them completely, and the rest could be detected by reporter cells, a condition called ‘posivive-biased pass’.

OK!

On the contrary, if the positions of amplifier cells and block cells in the equipment are reversed, the AHL produced by generator cell would be degraded by block cells at the very beginning resulting in no amplification of the signal. Thus, hardly AHL could be detected in the outflow culture, a condition called ‘negative-biased block’.

Blocked!

Model

1. Model design and construction

2. Model result

2.1 Model of Generator Cells

2.2 Model of Block Cells

2.3 Model of Amplifier Cells

2.4 Model of whole system

Experiment

1.Datas and Graphs of Detector cells

2.Function of immobilized cells

3.Assembly of equipment

@@ Line 7: / Line 7: @@
 <br><br>
-<font size="3" color="#ffff00" rowspan=2 > The diode, one of the most significant parts in the electric integrate circuit and electronic equipment, functions as allowing the current get through when the diode is positive-biased, and cut off while it is negative-biased. When the negative voltage increases to a threshold, the diode tends to be negatively broken down. In this project, the molecular signal AHL is considered as the current of electric circuit.Three types of cells, [[generator cell]]、[[amplifier cells]] and [[block cells]] are immobilized into small beads with a diameter of 0.5 millimeter, then different beads are settled into particular parts of our specially designed equipment sequentially and respectively. <br>Firstly, the liquid culture flow through the generator cells and carry the AHL signal into the amplifier cells which are activated to make more AHL signals. <br>Finally, the concentration of AHL accumulates to such a level that block cells could not eliminate them completely, and the rest could be detected by [[reporter cells]], a condition called ‘posivive-biased pass’.</font><br>
+<font size="3" color="#ffff00" rowspan=2 > The diode, one of the most significant parts in the electric integrate circuit and electronic equipment, functions as allowing the current get through when the diode is positive-biased, and cut off while it is negative-biased. When the negative voltage increases to a threshold, the diode tends to be negatively broken down. In this project, the molecular signal AHL is considered as the current of electric circuit.Three types of cells: [[generator cell]]、[[amplifier cells]] and [[block cells]] are immobilized into small beads with a diameter of 0.5 millimeter, then different beads are settled into particular parts of our specially designed equipment sequentially and respectively. <br>The liquid culture flow through the generator cells and carry the AHL signal into the amplifier cells which are activated to make more AHL signals. <br>Then the concentration of AHL accumulates to such a level that block cells could not eliminate them completely, and the rest could be detected by [[reporter cells]], a condition called ‘posivive-biased pass’.</font><br>
 [[Image:diodedh.gif|500px]]             <font size="8" color="#0000CC">OK!</font> [[Image:happy.gif|150px]]<br><br>
-<font size="3" color="#ffff00" rowspan=2 >On the contrary, if the positions of amplifier cells and block cells in the equipment are reversed, the AHL produced by generator cell would be degraded by block cells at the very beginning resulting in no amplification of the signal, thus, hardly AHL could be detected in the outflow culture, a condition called ‘negative-biased block’. </font>
+<font size="3" color="#ffff00" rowspan=2 >On the contrary, if the positions of amplifier cells and block cells in the equipment are reversed, the AHL produced by generator cell would be degraded by block cells at the very beginning resulting in no amplification of the signal. Thus, hardly AHL could be detected in the outflow culture, a condition called ‘negative-biased block’. </font>
 [[Image:diodeblock.gif|500px]]            <font size="8" color="#0000CC">Blocked!</font>[[Image:cry.gif]]<br><br><br><br>