Berkeley LBL/JoyceNotebook
From 2007.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
!''bchD'' | !''bchD'' | ||
!''bchI'' | !''bchI'' | ||
| + | |||
| + | |- | ||
| + | |length of gene fragment | ||
| + | |1695 b.p. | ||
| + | |1005 b.p. | ||
|- | |- | ||
| Line 12: | Line 17: | ||
|NdeI, BamHI | |NdeI, BamHI | ||
|NdeI, BglI | |NdeI, BglI | ||
| + | |||
| + | |- | ||
| + | | | ||
| + | | | ||
| + | | | ||
|} | |} | ||
Revision as of 19:26, 25 October 2007
Contruction of pET3a Derivatives Containing bchD and bchI
1. Amplify Rhodobacter's gene and introduce restriction sites by PCR (Using Phusion Polymerase), which allow cloning PCR fragments into pET3a
| bchD | bchI | |
|---|---|---|
| length of gene fragment | 1695 b.p. | 1005 b.p. |
| Restriction sites introduced when amplified by PCR | NdeI, BamHI | NdeI, BglI |
