Brown
From 2007.igem.org
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==Projects== | ==Projects== | ||
===Tri-stable Toggle Switch=== | ===Tri-stable Toggle Switch=== | ||
| - | The Tri-stable Toggle Switch will be able to produce three distinct, continuous (stable) outputs for each of the three inputs. A chemical will induce the system to "lock into" one state while repressing the other two states. Our three constructs are pBAD->LacI->TetR, pLacI->AraC->TetR, and pTet->AraC->LacI, where AraC represses pBAD, LacI represses pLac and TetR represses pTet. The three chemicals ([[http://en.wikipedia.org/wiki/Arabinose | + | The Tri-stable Toggle Switch will be able to produce three distinct, continuous (stable) outputs for each of the three inputs. A chemical will induce the system to "lock into" one state while repressing the other two states. Our three constructs are pBAD->LacI->TetR, pLacI->AraC->TetR, and pTet->AraC->LacI, where AraC represses pBAD, LacI represses pLac and TetR represses pTet. The three chemicals ([[http://en.wikipedia.org/wiki/Arabinose arabinose]], [[http://en.wikipedia.org/wiki/IPTG IPTG]] and [[http://en.wikipedia.org/wiki/Tetracycline Tetracycline]], respectively), cause conformational changes in their respective repressor proteins which leads to gene expression. For example, in the presence of arabinose, AraC cannot repress pBAD so LacI and TetR are produced which in turn repress pTet and pLac. |
The architecture of our system is as follows: | The architecture of our system is as follows: | ||
[[Image:Tristable_Toggle_Switch_2007.jpg|100px|frame|The Tri-stable Toggle Switch Architecture]] | [[Image:Tristable_Toggle_Switch_2007.jpg|100px|frame|The Tri-stable Toggle Switch Architecture]] | ||
Revision as of 17:24, 19 June 2007
Welcome to our World
Projects
Tri-stable Toggle Switch
The Tri-stable Toggle Switch will be able to produce three distinct, continuous (stable) outputs for each of the three inputs. A chemical will induce the system to "lock into" one state while repressing the other two states. Our three constructs are pBAD->LacI->TetR, pLacI->AraC->TetR, and pTet->AraC->LacI, where AraC represses pBAD, LacI represses pLac and TetR represses pTet. The three chemicals (http://en.wikipedia.org/wiki/Arabinose arabinose, http://en.wikipedia.org/wiki/IPTG IPTG and http://en.wikipedia.org/wiki/Tetracycline Tetracycline, respectively), cause conformational changes in their respective repressor proteins which leads to gene expression. For example, in the presence of arabinose, AraC cannot repress pBAD so LacI and TetR are produced which in turn repress pTet and pLac.
The architecture of our system is as follows:
