Imperial/Wet Lab/DNA Constructs
From 2007.igem.org
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|| || 25 || [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] || plux-GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font> || --- || [[Imperial/Infector_Detector/Design#DNA_Constructs|Infector Detector construct 2]] | || || 25 || [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] || plux-GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font> || --- || [[Imperial/Infector_Detector/Design#DNA_Constructs|Infector Detector construct 2]] | ||
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| - | || || 23 || [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] || GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font> || pT7 || | + | || || 23 || [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] || GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#0000FF>''Midi''</font> || pT7 || Used to construct [http://partsregistry.org/Part:BBa_I719015 BBa_I719015] |
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| - | || || || BBa_I719??? || DsRed Express || | + | || || || BBa_I719??? || DsRed Express || ''Delayed'' || ptet, plux, pT7 || The Visual reporter |
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| - | || || || BBa_I719??? || HrpR-HrpS || | + | || || || BBa_I719??? || HrpR-HrpS || ''Ready'' || ??? || Hrp regulators |
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| - | || || || BBa_I719??? || pHrpL || | + | || || || BBa_I719??? || pHrpL || ''Ready'' || ??? || Hrp promoter |
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| - | || || || BBa_I719??? || HrpV || | + | || || || BBa_I719??? || HrpV || ''Ready'' || ??? || Hrp inhibitor system |
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|width="--"%|Application | |width="--"%|Application | ||
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| - | || || || | + | || || || [http://partsregistry.org/Part:BBa_I719015 BBa_I719015] || pT7 & GFP || <font color=#CC0000>XL1</font>, <font color=#CC00FF>''Maxi''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font> || pT7 + [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] || [[Imperial/Cell_by_Date/Design#DNA_Constructs|Cell By Date concept 2]] |
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| - | || || || BBa_I719??? || pT7 & DsRedEx || Planned || pT7 + DsRedEx || | + | || || || BBa_I719??? || pT7 & DsRedEx || Planned || pT7 + DsRedEx || [[Imperial/Cell_by_Date/Design#DNA_Constructs|Cell By Date concept 2]] with visual reporter |
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| - | || || || BBa_I719??? || ptet & DsRedEx || Planned || [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] + DsRedEx || | + | || || || BBa_I719??? || ptet & DsRedEx || Planned || [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] + DsRedEx || [[Imperial/Cell_by_Date/Design#DNA_Constructs|Cell By Date concept 1]] with visual reporter |
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| - | || || || BBa_I719??? || ptet-luxR-plux-DsRedEx || Planned || [http://partsregistry.org/Part:BBa_T2620 BBa_F2620] + DsRedEx || | + | || || || BBa_I719??? || ptet-luxR-plux-DsRedEx || Planned || [http://partsregistry.org/Part:BBa_T2620 BBa_F2620] + DsRedEx || [[Imperial/Infector_Detector/Design#DNA_Constructs|Infector Detector construct 1]] with visual reporter |
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| - | || || || BBa_I719??? || plux-DsRedEx || Planned || [http://partsregistry.org/Part:BBa_R0062 BBa_R0062] + DsRedEx || | + | || || || BBa_I719??? || plux-DsRedEx || Planned || [http://partsregistry.org/Part:BBa_R0062 BBa_R0062] + DsRedEx || [[Imperial/Infector_Detector/Design#DNA_Constructs|Infector Detector construct 2]] with visual reporter |
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| - | || A || || 26 || BBa_S03119 || ptet-LuxR || pSB1A2 || XL1, Midi | + | || A || || 26 || BBa_S03119 || ptet-LuxR || pSB1A2 || XL1, Midi || 998bp || not used in design |
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Revision as of 06:58, 26 October 2007
Wet Lab: DNA Constructs
Constructs Overview
Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details.
Cloning Table
Status of biobricks cloned and their applications:
| I | ID | Part | Description | Status | Ligation Target | Use in Constructs |
| 02 | [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] | ptet-GFP | XL1, Maxi, Mini, BL21 | --- | Cell By Date concept 1 | |
| 11 | [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] | pT7 GFP | XL1, Maxi, Midi, Mini, BL21 | --- | Cell By Date concept 2 | |
| 30 | --- | pT7 (strong GFP) | XL1, Maxi, Mini, BL21 | --- | Construct to test T7 promoter | |
| 18 | [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | ptet-luxR-plux-GFP | XL1, Maxi, Midi, BL21 | --- | Infector Detector construct 1 | |
| 05 | [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | ptet | XL1, Midi, Mini | DsRedEx | Cell By Date concept 1 with visual reporter | |
| [http://partsregistry.org/Part:BBa_I719005 BBa_I719005] | pT7 | Midi | DsRedEx | Cell By Date concept 2 | ||
| 07 | [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] | ptet-luxR-plux | XL1, Midi, Mini | DsRedEx | Infector Detector construct 1 with visual reporter | |
| 21 | [http://partsregistry.org/Part:BBa_J23039 BBa_J23039] | ptet-luxI | XL1, Midi | --- | AHL sender construct | |
| 24 | [http://partsregistry.org/Part:BBa_R0062 BBa_R0062] | plux | XL1, Midi | DsRedEx | Infector Detector construct 2 with visual reporter | |
| 25 | [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] | plux-GFP | XL1, Maxi, Midi | --- | Infector Detector construct 2 | |
| 13 | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | Double terminator | XL1, Maxi, Midi, Mini | --- | General part construction | |
| 17 | [http://partsregistry.org/Part:BBa_I13507 BBa_I13507] | RFP | XL1, Midi | --- | Not being used anymore | |
| 19 | [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | strong RBS | XL1 | --- | General part construction | |
| 23 | [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] | GFP | XL1, Maxi, Midi | pT7 | Used to construct [http://partsregistry.org/Part:BBa_I719015 BBa_I719015] | |
| BBa_I719??? | DsRed Express | Delayed | ptet, plux, pT7 | The Visual reporter | ||
| BBa_I719??? | HrpR-HrpS | Ready | ??? | Hrp regulators | ||
| BBa_I719??? | pHrpL | Ready | ??? | Hrp promoter | ||
| BBa_I719??? | HrpV | Ready | ??? | Hrp inhibitor system | ||
Ligation Table
Status of parts ligated and their applications:
| I | ID | Part | Description | Status | Parents | Application |
| [http://partsregistry.org/Part:BBa_I719015 BBa_I719015] | pT7 & GFP | XL1, Maxi, Mini, BL21 | pT7 + [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] | Cell By Date concept 2 | ||
| BBa_I719??? | DsRedEx & pSB1A3 | Paused | DsRedEx + [http://partsregistry.org/Part:BBa_P1010 BBa_P1010] | Biobrick Submission | ||
| BBa_I719??? | pT7 & DsRedEx | Planned | pT7 + DsRedEx | Cell By Date concept 2 with visual reporter | ||
| BBa_I719??? | ptet & DsRedEx | Planned | [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] + DsRedEx | Cell By Date concept 1 with visual reporter | ||
| BBa_I719??? | ptet-luxR-plux-DsRedEx | Planned | [http://partsregistry.org/Part:BBa_T2620 BBa_F2620] + DsRedEx | Infector Detector construct 1 with visual reporter | ||
| BBa_I719??? | plux-DsRedEx | Planned | [http://partsregistry.org/Part:BBa_R0062 BBa_R0062] + DsRedEx | Infector Detector construct 2 with visual reporter | ||
Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Maxi - maxiprepped; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3); status - stocks running low]
Parts from the Registry
Physical information of biobricks:
| A | W | G | S | ID | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(T) | Location | Conc. (ng/ul) |
| A | W | G | 02 | [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] | GFP under tetracyclin promoter | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 937bp | 3016bp | Well 15H, Plate 2 | 500 | |
| A | W | G | 05 | [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | tetracyclin promoter | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 54bp | 2133bp | Well 7O, Plate 1 | 60 | |
| A | W | G | 07 | [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] | lux promoter, with luxR | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 1061bp | 3140bp | Well 6B, Plate 1 | 80 | |
| A | X | G | 11 | [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] | T7 GFP | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 826bp | 2905bp | Well 13F, Plate 2 | 100 | |
| A | W | G | 13 | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | Double terminator | [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] | A+KR | High | 129bp | 3316bp | Well 1B, Plate 2 | 100/70 | |
| A | G | 17 | [http://partsregistry.org/Part:BBa_I13507 BBa_I13507] | RFP | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 861bp | 2940bp | Well 16N, Plate 1 | 80 | ||
| A | W | G | 18 | [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | GFP under lux promoter with LuxR | [http://partsregistry.org/Part:pSB1A3 pSB1A3] | AmpR | High | 1945bp | 4102bp | Well 19B, Plate 2 | 460 | |
| A | W | 19 | [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | RBS | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 12bp | 2091bp | Well 3O, Plate 1 | -- | ||
| A | G | 21 | [http://partsregistry.org/Part:BBa_J23039 BBa_J23039] | Constitutive luxI | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 860bp | 2939bp | Well 11K, Plate 3 | 140 | ||
| A | W | G | 23 | [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] | GFP | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 875bp | 2954bp | Well 16E, Plate 1 | 120/330 | |
| A | G | 24 | [http://partsregistry.org/Part:BBa_R0062 BBa_R0062] | lux promoter | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 55bp | 2134bp | Well 9G, Plate 1 | 90 | ||
| A | G | 25 | [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] | GFP under lux promoter | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | AmpR | High | 938bp | 3017bp | Well 8D, Plate 3 | 125/330 | ||
| W | 30 | --- | T7 with GFP(strong) | --- | Kan? | ??? | --- | ??? | --- | 150 | |||
Our Parts
Physical information of our ligated parts:
| A | W | G | S | ID | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(P) | Stock |
| [http://partsregistry.org/Part:BBa_I719022 BBa_I719022] | cI with GFP | pSB1A2 | AmpR | High | 933bp | 3988bp | ||||||
| BBa_I791021 | luxI coding region under ptet | pSB1A2 | AmpR | High | 860bp | 2939bp | ||||||
| BBa_I791??? | DsRed Express under ptet | pSB1A2 | AmpR | High | ||||||||
Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced)
Concentrations are given in ng/μl
Part Graveyard
General information on parts we do not like...
| A | W | ID | Name | Description | Plasmid | Size | Status | Notes |
| A | W | 01 | BBa_E0040 | GFP | pSB1A2 | 720bp | XL1, Mini | Redundant |
| A | 03 | BBa_J5528 | pBad-GFP | pSB2K3 | 2093bp | failed-XL1 | ara construct abandoned | |
| A | 04 | BBa_J24677 | plux-GFP | pSB1A2 | 1787bp | XL1, Mini | Wrong GFP ligated | |
| A | 06 | BBa_I0500 | pBad | pSB2K3 | 1210bp | XL1, Mini, Midi | ara construct abandoned | |
| A | W | 08 | BBa_F1610 | luxI | pSB1AK3 | 798bp | XL1, Mini, Midi | Substitute part J23039 |
| A | W | 09 | BBa_R0010 | lacI-plac | pSB1A2 | 200bp | XL1, Mini | Lac construct abandoned |
| A | W | 10 | BBa_E1010 | RFP | pSB2K3 | 681bp | XL1, Mini | Substitute part I13507 |
| A | 12 | BBa_S03550 | araC | pSB1AC3 | 958bp | XL1, Mini, Midi | araC construct abandoned | |
| W | 14 | BBa_R0051 | pcI | pSB1A2 | 49bp | Biobrick | pcI construct abandoned | |
| A | 15 | BBa_J07037 | cI-GFP | pSB2K3 | 912bp | XL1, Mini | No RBS present on this part | |
| A | W | 16 | BBa_E0240 | GFP | pSB1A2 | 876bp | XL1, Midi | Redundant |
| W | 20 | --- | AraC | pSB1AC3 | 1095bp | Planned | araC construct abandoned | |
| A | 26 | BBa_S03119 | ptet-LuxR | pSB1A2 | XL1, Midi | 998bp | not used in design | |
| A | 27 | pSB1A3 | pSB1A3 | pSB1A3 | failed-XL1 | 2157bp | biobrick not present | |
| A | W | 28 | BBa_P1010 | pSB1A3 (ccd) | pSB1A3 | 0bp | failed-XL1 | Strain not available |
| A | W | 29 | BBa_P1010 | pSB1A2 (ccd) | pSB1A2 | 0bp | --- | Strain not available |
