From 2007.igem.org
(Difference between revisions)
|
|
| Line 5: |
Line 5: |
| | [[Image:TJUcolumn1.jpg|400px]]<br> | | [[Image:TJUcolumn1.jpg|400px]]<br> |
| | ==Test Result== | | ==Test Result== |
| - | All the result of fluorescence values is based on the function of our detector cell which is shown [[Tianjin/DIODE/Experiment|here]].<br>
| + | |
| | + | The datas of fluorescence intensity are derived from the mixture of detector cells with |
| | + | |
| | + | [[Tianjin/DIODE/Experiment|here]].<br> |
| | | | |
| | Figure 1: | | Figure 1: |
| - | We test the equipment containing the generator cell and the block cell at different flux and different time after the flux was changed.The result shows that the output concentration of AHL will go down when the flux increased and the block cell will degradate the AHL produced by the generator cell but still a bit higher than we expected.<br> | + | We test the equipment fulfulling with the generator cell and the block cell at different flux rate and different time after the alteration of flux rate.The result shows that the output concentration of AHL will decrease with the increase of the flux rates and the block cells could degradate the AHL produced by the generator cell,but the value is still a little higher than what we expected.<br> |
| | [[Image:tjuex301.jpg|500px]]<br> | | [[Image:tjuex301.jpg|500px]]<br> |
| | <br> | | <br> |
| Line 16: |
Line 19: |
| | <br> | | <br> |
| | <br> | | <br> |
| - | Figure 3:We test the postive-biased and the negative-biased condition of the system .The result show that the block cell cause the concentration of AHL get down.We can observe that the fluorescence value of postive-biased and negative-biased condition are almost the same which reveals that the amplifier cells are opened at both condition .When we add IPTG,the fluorescence of postive-biased will reach a hihi valuev as we expected. <br> | + | Figure 3:We test the postive-biased and the negative-biased condition of the system .The result shows that the block cell successfully reduces the concentration of AHL.We can observe that the fluorescence value of postive-biased and negative-biased condition are almost the same which reveals that the amplifier cells are working at both condition .When we add IPTG,the fluorescence of postive-biased will reach a high value as we expected. <br> |
| | [[Image:tjuex303.jpg|500px]]<br> | | [[Image:tjuex303.jpg|500px]]<br> |
| | <br> | | <br> |
| | <br> | | <br> |
| - | Figure 4：We test generator cells together with the amplifier cells at flux=55.6ul/s .The result show that the concentration of AHL get higher when amplifier cells was added and the IPTG was added.<br> | + | Figure 4：We test generator cells together with the amplifier cells at flux rate of 55.6ul/s .The result shows that the concentration of AHL become higher when amplifier cells together with IPTG were added.<br> |
| | [[Image:tjuex304.jpg|500px]]<br> | | [[Image:tjuex304.jpg|500px]]<br> |
| | | | |
| | | | |
| | |} | | |} |
Revision as of 09:54, 26 October 2007
Introduction
Equipment
Test Result
The datas of fluorescence intensity are derived from the mixture of detector cells with
here.
Figure 1:
We test the equipment fulfulling with the generator cell and the block cell at different flux rate and different time after the alteration of flux rate.The result shows that the output concentration of AHL will decrease with the increase of the flux rates and the block cells could degradate the AHL produced by the generator cell,but the value is still a little higher than what we expected.
Figure 2:We put in more block cells than that in figure 1,that is 1ml immobilized generator cells and 4ml immobilized block cells .The result shows that the block cells also work but not as good as we want.
Figure 3:We test the postive-biased and the negative-biased condition of the system .The result shows that the block cell successfully reduces the concentration of AHL.We can observe that the fluorescence value of postive-biased and negative-biased condition are almost the same which reveals that the amplifier cells are working at both condition .When we add IPTG,the fluorescence of postive-biased will reach a high value as we expected.
Figure 4：We test generator cells together with the amplifier cells at flux rate of 55.6ul/s .The result shows that the concentration of AHL become higher when amplifier cells together with IPTG were added.
|
