Imperial/Wet Lab/Lab Notebook/2007-08-13
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**BBa_E7104 [T7-GFP]</font> | **BBa_E7104 [T7-GFP]</font> | ||
| - | Protocols can be found at [ | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis | Electroporation] in the general protocols page |
==Pilot Preparation of Vesicles== | ==Pilot Preparation of Vesicles== | ||
Revision as of 12:40, 26 October 2007
13 August 2007
Preparation of Stock Solutions
- 1x 1 litre of 2xYT medium in Duran bottle
- 3x 1 litre of 2xYT medium in conical flask
- Sent to autoclave
- Solution A for preparation of vesicles
Transformation of Biobricks
- 7 biobrick were transformed into XL1-Blue or BL21(DE3)
- Cells were grown for 1 hour and plated
- Plates were incubated at 37°C overnight
- Transformed in XL1-Blue
- BBa_J5528 [pBad-GFP] (re-clone)
- BBa_E0240 [GFP]
- BBa_I13507 [RFP]
- BBa_T9002 [ptet-luxR-pLux-GFP]
- BBa_B0034 [RBS]
- BBa_I13522 [ptet-GFP]
- BBa_E7104 [T7-GFP]
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis | Electroporation] in the general protocols page
Pilot Preparation of Vesicles
- Followed the desiccation and suspension stages of the vesicle preparation protocol
- One 100ml beaker was prepared with 125μl of DOPC in 50ml of mineral oil
- The suspension was sonicated for 30mins and left overnight in a 27°C incubator
