Melbourne/Lab Notebook gv F1
From 2007.igem.org
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==Example pNL29(orininal plasmid) and Tube(64) the IPTG promoted version of BBa_I750016== | ==Example pNL29(orininal plasmid) and Tube(64) the IPTG promoted version of BBa_I750016== | ||
| + | Image:Melbourne-float1 11am 18-10-2007.JPG | ||
Revision as of 13:29, 26 October 2007
Testing Floatation
- Growing up cells from glycerol stocks overnight in 5mL LB amp
- Prepare 250ml conical shaker flasks by cleaning and autoclave.
- Add 50mL of LB Amp to each of two flasks.
- Add 1M IPTG to one flask to make final concentration of 1mM IPTG, Lable with I.
- Transfer 1mL of overnight culture to each flask.
- Grow overnight at 37degC on a shaker.
- Next morning transfer to 50ml falcon tubes.
- Spin down the cells gently 25minutes at 1000rpm in Eppendorph 5810R centrifuge.
- Poor off and discard the LB, maintaining the pellet and a residual ammount of LB 1ml about.
- Replace LB with 50ml of 10g/L NaCl filtered through a 0.22um filter.
- Resuspend the cells by shaking.
- Leave at room temperature and observe the settling over the next week.
Example pNL29(orininal plasmid) and Tube(64) the IPTG promoted version of BBa_I750016
Image:Melbourne-float1 11am 18-10-2007.JPG
