PennState/Parts

From 2007.igem.org

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<a href="http://partsregistry.org/Part:BBa_I741005:Experience"><img border="0" src="http://openwetware.org/images/3/3e/Psuigem07picGene.jpg" width="121" height="121"></a></td>
<a href="http://partsregistry.org/Part:BBa_I741005:Experience"><img border="0" src="http://openwetware.org/images/3/3e/Psuigem07picGene.jpg" width="121" height="121"></a></td>
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<td class="tddesc"><b><a href="http://partsregistry.org/Part:BBa_I741005">xylR</a></b><p><i>XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.</i></td>
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<td class="tddesc"><b><a href="http://partsregistry.org/Part:BBa_I741005">xylR</a></b><p><i>XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.</i><a href="http://biocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG20253-MONOMER">[BioCyc.org]</a></td>
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<a href="http://partsregistry.org/Part:BBa_I741018:Experience"><img border="0" src="http://openwetware.org/images/2/29/Psuigem07picPromoteroneway.jpg" width="121" height="121"></a></td>
<a href="http://partsregistry.org/Part:BBa_I741018:Experience"><img border="0" src="http://openwetware.org/images/2/29/Psuigem07picPromoteroneway.jpg" width="121" height="121"></a></td>
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<td class="tddesc"><b><a href="http://partsregistry.org/Part:BBa_I741018">Promoter xylF</a></b><p><i>The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR.  Two xylR halfsites are included as well as a CRP activator binding site.</i></td>
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<td class="tddesc"><b><a href="http://partsregistry.org/Part:BBa_I741018">Promoter xylF</a></b><p><i>The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR.  Two xylR halfsites are included as well as a CRP activator binding site.</i><a href="http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00374">[BioCyc.org]</a></td>
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Revision as of 20:43, 26 October 2007

Home Diauxie Project Dosimeter Project People Lab Parts Acknowledgements Links


New Parts (submitted)

xylR

XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.[BioCyc.org]

Promoter xylF

The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.[BioCyc.org]



New Parts (underconstructed/unverified)

natural RBS xylR TT 'xylR total'

XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.

CRP*

CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.[Hanamura92]

Promoter xylA

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.

Promoter xylA without CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working.

Promoter xylF without CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working.

Two way promoter xylA-F with CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included and a CRP binding site.



References

Hanamura92: Hanamura A, Aiba H (1992). "A new aspect of transcriptional control of the Escherichia coli crp gene: positive autoregulation." Mol Microbiol 1992;6(17);2489-97. PMID: 1328816