From 2007.igem.org
< PennState | Project | Diauxie(Difference between revisions)
|
|
| Line 1: |
Line 1: |
| | '''Initial Setup''' | | '''Initial Setup''' |
| - |
| |
| - | To test each of the four xyl promoters, each is placed in a construct followed by a ribosome binding site, the reporter green florescent protein, and a terminator. The strength of each is measured by gfp emission.
| |
| - |
| |
| - |
| |
| - | <html><img src="http://openwetware.org/images/2/2a/Fptest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylF facing promoter that includes the CRP binding region.
| |
| - |
| |
| - |
| |
| - | <html><img src="http://openwetware.org/images/d/da/Aptest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing promoter that includes the CRP binding region.
| |
| - |
| |
| - |
| |
| - |
| |
| - | <html><img src="http://openwetware.org/images/b/b1/Fpnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing promoter that does not include the CRP binding region.
| |
| - |
| |
| - |
| |
| - |
| |
| - | <html><img src="http://openwetware.org/images/5/57/Apnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing promoter that does not include the CRP binding region.
| |
Latest revision as of 21:09, 26 October 2007
Initial Setup
