PennState/Project/Diauxie/PromoterTest
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| - | <html><img src="http://openwetware.org/images/ | + | <html><img src="http://openwetware.org/images/8/88/Pctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylF and xylA facing promoters that includes the CRP binding region. |
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<html><img src="http://openwetware.org/images/b/b1/Fpnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing promoter that does not include the CRP binding region. | <html><img src="http://openwetware.org/images/b/b1/Fpnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing promoter that does not include the CRP binding region. | ||
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After each of the four promoter constructs are tested individually, the two | After each of the four promoter constructs are tested individually, the two | ||
Revision as of 21:21, 26 October 2007
Promoter Test Constructs
To test each of the four xyl promoters, each is placed in a construct followed by a ribosome binding site, the reporter green florescent protein, and a terminator. The strength of each is measured by gfp emission.
This construct uses the xylF and xylA facing promoters that includes the CRP binding region.
This construct uses the xylA facing promoter that does not include the CRP binding region.
After each of the four promoter constructs are tested individually, the two
