Vincent Parker Notebook
From 2007.igem.org
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[[User:JEDueber|JEDueber]] 16:02, 25 June 2007 (EDT) | [[User:JEDueber|JEDueber]] 16:02, 25 June 2007 (EDT) | ||
| - | Today i came into the lab and found that 3 out of the 4 PCR tubes were in the freezer but i have lost the tube marked V6-8. i had the PCR products from the tubes V1-3, V2-4, and V5-7. | + | Today i came into the lab and found that 3 out of the 4 PCR tubes were in the freezer but that i have lost the tube marked V6-8. i had the PCR products from the tubes V1-3, V2-4, and V5-7. After having fumed a bit over my lost PCR i went to work on setting up a new PCR tube for V6-8 and to also clean tubes V1-3, V2-4 and V5-7. Austin was kind enough to share the PCR machine with me and i put in the tube V6-8 in and also the sewing reaction i was working on named V1-4. V1-4 is a combination between V1-3 and V2-4. Since V1-3 and V2-4 are two parts of one gene and i had to PCR out the internal restriction site. |
Revision as of 20:25, 25 June 2007
JEDueber 16:02, 25 June 2007 (EDT) Today i came into the lab and found that 3 out of the 4 PCR tubes were in the freezer but that i have lost the tube marked V6-8. i had the PCR products from the tubes V1-3, V2-4, and V5-7. After having fumed a bit over my lost PCR i went to work on setting up a new PCR tube for V6-8 and to also clean tubes V1-3, V2-4 and V5-7. Austin was kind enough to share the PCR machine with me and i put in the tube V6-8 in and also the sewing reaction i was working on named V1-4. V1-4 is a combination between V1-3 and V2-4. Since V1-3 and V2-4 are two parts of one gene and i had to PCR out the internal restriction site.
