From 2007.igem.org

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	4. Incubate approximately 18hrs in 37 degree shaker		4. Incubate approximately 18hrs in 37 degree shaker
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Latest revision as of 04:22, 27 June 2007

1. To 5ml LB add relevant amount of selective antibiotic

2. Flame pipette tip and pick single colony from plate

3. Introduce bacteria to LB

4. Incubate approximately 18hrs in 37 degree shaker

Protocols