Berkeley LBL/Results

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[[Berkeley_LBL/Results/ProteinGel|SDS-PAGE Gel of Soluble Proteins]]
[[Berkeley_LBL/Results/ProteinGel|SDS-PAGE Gel of Soluble Proteins]]
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== Results ==
 
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[[Berkeley_LBL/Ladder|Invitrogen BenchMark Protein Ladder]]
 
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[[Berkeley_LBL/Key|Plasmid Key for Protein Gels]]
 
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[[Image:ProteinGel1.jpg]]
 
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[[Image:Gel1Key.jpg]]
 
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[[Image:ProteinGel2.jpg]]
 
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[[Image:Gel2Key.jpg]]
 
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[[Image:ProteinGel3.jpg]]
 
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[[Image:Gel3Key.jpg]]
 
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[[Image:ProteinGel4.jpg]]
 
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[[Image:Gel4Key.jpg]]
 
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== Discussion ==
 
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Prior to running the protein gels, we expected to see protein bands for the constructs that contained the -H and -I gene(~140kDa and ~38kDa, respectively) and were also induced with IPTG .  We did not expect to see bands that coded for the -D gene (~70kDa) and for all constructs that were not induced with IPTG.  Although the induced constructs that contained the -H gene had strong bands at ~140kDa, the uninduced constructs also showed strong bands in the same area.  This gene was able to be expressed with or without induction of IPTG.  In addition, protein bands that coded for the -I gene showed a strong band; however, when expressed with the -H gene, it did not clearly show a strong band at ~38 kDa in comparison to the benchmark ladder.  As expected, protein bands for the -D gene also did not show, alone nor in conjunction with the -H and -I gene.  Although our protein gels did not convey expression of neither the -I nor -D genes, our genes may still be expressed and may have enough activity to catalyze the Mg-chelatase enzyme to produce Mg-protoporphyrin IX.
 

Revision as of 02:56, 27 October 2007

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SDS-PAGE Gel of Soluble Proteins

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