Princeton
From 2007.igem.org
| Line 7: | Line 7: | ||
=[[Princeton/Project Description | An RNAi-Enhanced Logic Circuit: Cancer-Specific Detection and Destruction]]= | =[[Princeton/Project Description | An RNAi-Enhanced Logic Circuit: Cancer-Specific Detection and Destruction]]= | ||
| - | The [[Princeton/People | Princeton University iGEM 2007 team]], consisting of 10 undergraduate students, 2 high school students, and | + | The [[Princeton/People | Princeton University iGEM 2007 team]], consisting of 10 undergraduate students, 2 high school students, and 7 instructors, envisions a paradigm shift in the way one can target cancer and destroy the resulting cancerous cells. |
Using standard engineering practices, we have designed, and are in the process of implementing, a novel system that utilizes RNA interference (RNAi) to detect and destroy cancer cells in a tissue-specific manner. We are interfacing RNAi components together with promoter and repressor elements to form logic circuits, which allow the use of multiple criteria to target cancerous cells in a unique and highly specific manner. As a measure of safety, we utilize mutant lentiviral integrase to deliver our construct into the cell as a non-integrated plasmid, preventing any disruptive effects that could be attributed to pseudorandom integration by the lentivirus. This also ensures that the daughter cells will not inherit the cancer-detecting circuitry, thus preventing unintended proliferation of our construct and allow for more extensive and comprehensive cancer treatments. | Using standard engineering practices, we have designed, and are in the process of implementing, a novel system that utilizes RNA interference (RNAi) to detect and destroy cancer cells in a tissue-specific manner. We are interfacing RNAi components together with promoter and repressor elements to form logic circuits, which allow the use of multiple criteria to target cancerous cells in a unique and highly specific manner. As a measure of safety, we utilize mutant lentiviral integrase to deliver our construct into the cell as a non-integrated plasmid, preventing any disruptive effects that could be attributed to pseudorandom integration by the lentivirus. This also ensures that the daughter cells will not inherit the cancer-detecting circuitry, thus preventing unintended proliferation of our construct and allow for more extensive and comprehensive cancer treatments. | ||
| Line 18: | Line 18: | ||
|-valign="top" | |-valign="top" | ||
|width=179.25px style="padding: 5px; background-color: #ffffaa; border: 2px solid #993300;" | | |width=179.25px style="padding: 5px; background-color: #ffffaa; border: 2px solid #993300;" | | ||
| - | |||
| - | |||
<h3>Team Members</h3> | <h3>Team Members</h3> | ||
| Line 41: | Line 39: | ||
*[[user:ilemisch|Ihor Lemischka]] [http://directory.mssm.edu/faculty/facultyInfo.php?id=43268&deptid=92 Website] | *[[user:ilemisch|Ihor Lemischka]] [http://directory.mssm.edu/faculty/facultyInfo.php?id=43268&deptid=92 Website] | ||
*[[user:cry707|Christoph Schaniel]] [http://directory.mssm.edu/faculty/facultyInfo.php?id=43270&deptid=92 Website] | *[[user:cry707|Christoph Schaniel]] [http://directory.mssm.edu/faculty/facultyInfo.php?id=43270&deptid=92 Website] | ||
| - | + | *[[user:pguye|Patrick Guye]] | |
| + | *[[user:eskamoah|Noah Davidsohn]] | ||
| + | *[[user:saurabhg|Saurabh Gupta]] | ||
|width=179.25px style="padding: 5px; background-color: #ffffaa; border: 2px solid #993300;" | | |width=179.25px style="padding: 5px; background-color: #ffffaa; border: 2px solid #993300;" | | ||
| + | <h3>Lab Work</h3> | ||
| + | [[Princeton/lab | Lab work]] | ||
| + | <h3>Research</h3> | ||
| + | [[Princeton/lab/experimentation | Experimentation]] | ||
| + | |||
| + | <h3>Bioinformatics</h3> | ||
| + | [[Princeton/lab/bioinformatics | Bioinformatics]] | ||
| + | |||
| + | <h3>Simulations</h3> | ||
| + | [[Princeton/lab/simulation | Simulations]] | ||
| + | |||
| + | <h3>Pictures</h3> | ||
| + | [[Princeton/People | Our happy team]] | ||
| - | |||
|width=179.25px style="padding: 5px; background-color: #ffffaa; border: 2px solid #993300;" | | |width=179.25px style="padding: 5px; background-color: #ffffaa; border: 2px solid #993300;" | | ||
<h3>Resources</h3> | <h3>Resources</h3> | ||
| - | + | [[Princeton/literature | literature]] | |
| - | + | ||
| - | + | ||
Revision as of 03:14, 27 October 2007
An RNAi-Enhanced Logic Circuit: Cancer-Specific Detection and DestructionThe Princeton University iGEM 2007 team, consisting of 10 undergraduate students, 2 high school students, and 7 instructors, envisions a paradigm shift in the way one can target cancer and destroy the resulting cancerous cells. Using standard engineering practices, we have designed, and are in the process of implementing, a novel system that utilizes RNA interference (RNAi) to detect and destroy cancer cells in a tissue-specific manner. We are interfacing RNAi components together with promoter and repressor elements to form logic circuits, which allow the use of multiple criteria to target cancerous cells in a unique and highly specific manner. As a measure of safety, we utilize mutant lentiviral integrase to deliver our construct into the cell as a non-integrated plasmid, preventing any disruptive effects that could be attributed to pseudorandom integration by the lentivirus. This also ensures that the daughter cells will not inherit the cancer-detecting circuitry, thus preventing unintended proliferation of our construct and allow for more extensive and comprehensive cancer treatments. We have constructed many components of our system and are in the process of testing our plasmids in a proof of concept manner. Where possible, we will revise and improve upon our components based on mathematical modeling and experimental results. Lab work, including experimentation, bioinformatics, and simulations, and literature have been used as feedback mechanisms to improve the implementation of the designed systems. Please see the extended overview for more information. |
Team Members
Instructors
|
Lab WorkResearchBioinformaticsSimulationsPictures |
Resourcesliterature |
