Chase Simulator
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= Project 2 Chase Simulator = | = Project 2 Chase Simulator = | ||
| - | * The first idea of project was to simulate the competition between two different warrior cell types made from E. coli to invade a semi-solid plate having a lawn of passive cells. The green and red cells are the invaders, and they represent two teams. The 'empty cells' are the ones to be invaded. Empty cells have two constructs and each code for a fluorescent protein and an inhibitor. They are normally off. One of the constructs (coding for green) can be activated by nearby green cells and the other(coding for red) by nearby red cells. Once one of the constructs in the empty cells is activated by an invader, expression of this construct irreversibly prevents activation of the other and the cell becomes permanatly green or permenantly red. The aim was to have a sort of game, where red cells and green cells would be competing to convert to empty cells so that they would not affected by the opponent cell any more. | + | * The first idea of project was to simulate the competition between two different warrior cell types made from E. coli to invade a semi-solid plate having a lawn of passive cells. The green and red cells are the invaders, and they represent two teams. The 'empty cells' are the ones to be invaded. Empty cells have two constructs and each code for a fluorescent protein and an inhibitor. They are normally off. One of the constructs (coding for green) can be activated by nearby green cells and the other(coding for red) by nearby red cells, possibly through quorum sensing molecules. Once one of the constructs in the empty cells is activated by an invader, expression of this construct irreversibly prevents activation of the other and the cell becomes permanatly green or permenantly red. The aim was to have a sort of game, where red cells and green cells would be competing to convert to empty cells so that they would not affected by the opponent cell any more. |
* The game could be fun, if 2 people separetely inoculate an area of their own choice on the same plate having a lawn of empty cells, with same amount of green and red invader cells each. The winner could be determined after incubation, by using fluorescence to see whose cells have invaded more of the plate. To add some challange, plates with different topologies (mountains or seas could be made ont the agar plate) could be prepared so choosing the area of inoculation would become a strategical decision. | * The game could be fun, if 2 people separetely inoculate an area of their own choice on the same plate having a lawn of empty cells, with same amount of green and red invader cells each. The winner could be determined after incubation, by using fluorescence to see whose cells have invaded more of the plate. To add some challange, plates with different topologies (mountains or seas could be made ont the agar plate) could be prepared so choosing the area of inoculation would become a strategical decision. | ||
Latest revision as of 04:14, 27 October 2007
Project 2 Chase Simulator
- The first idea of project was to simulate the competition between two different warrior cell types made from E. coli to invade a semi-solid plate having a lawn of passive cells. The green and red cells are the invaders, and they represent two teams. The 'empty cells' are the ones to be invaded. Empty cells have two constructs and each code for a fluorescent protein and an inhibitor. They are normally off. One of the constructs (coding for green) can be activated by nearby green cells and the other(coding for red) by nearby red cells, possibly through quorum sensing molecules. Once one of the constructs in the empty cells is activated by an invader, expression of this construct irreversibly prevents activation of the other and the cell becomes permanatly green or permenantly red. The aim was to have a sort of game, where red cells and green cells would be competing to convert to empty cells so that they would not affected by the opponent cell any more.
- The game could be fun, if 2 people separetely inoculate an area of their own choice on the same plate having a lawn of empty cells, with same amount of green and red invader cells each. The winner could be determined after incubation, by using fluorescence to see whose cells have invaded more of the plate. To add some challange, plates with different topologies (mountains or seas could be made ont the agar plate) could be prepared so choosing the area of inoculation would become a strategical decision.
- The important point in this project is to have two promoters which are normally off, and also repressed and activated by distinct proteins. There should be no leakage, because a little leakage in one of the constructs inhibits the other irreversibly. As there are not many of such non-leaking promoters, we created a less complex version;
- We reduced the invaders to one type, and increased the number of plates to two that each player would be using a separate plate this time using the same type of inveer cells. This invader would activate a normally off promoter which codes for a flourescent protein. In the registry we found three promoter-activator pairs for this purpose. However they do not have experimental information, so we do not know if they work properly.
- By this single invader cell type method, we can overcome the problems caused by leaky promoters. If there is a constitutive leakage, there will be a low level expression of the fluorescent protein even when it is not invaded, but we can make a base level correction to see the resulting effect or contribution of invader to the expression of the fluorescent protein by the empty cells.
- The three promoter-activator pairs are:
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style='text-align:center'><b style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:14.0pt'>Plate<o:p></o:p></span></b></p> </td> <td width=67 valign=top style='width:40.15pt;border:solid windowtext 1.0pt; border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:14.0pt'>Well<o:p></o:p></span></b></p> </td> <td width=101 valign=top style='width:60.85pt;border:solid windowtext 1.0pt; border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:14.0pt'>Plasmid<o:p></o:p></span></b></p> </td> <td width=158 valign=top style='width:94.8pt;border:solid windowtext 1.0pt; border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:14.0pt'>Length<o:p></o:p></span></b></p> </td> <td width=83 valign=top style='width:49.5pt;border:solid windowtext 1.0pt; border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:14.0pt'>Cell<o:p></o:p></span></b></p> </td> <td width=59 valign=top style='width:35.15pt;border:solid windowtext 1.0pt; border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:14.0pt'>Sel.<o:p></o:p></span></b></p> </td> </tr> <tr style='mso-yfti-irow:1'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US>BBa_B0015<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>Double terminator</span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>1</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>1I</span></p> </td> <td width=101 style='width:60.85pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center;line-height:150%'><span lang=EN-US>pSB1AK3</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>3189 3318 445</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>V1001</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>AK</span></p> </td> </tr> <tr style='mso-yfti-irow:2'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US>BBa_B0015<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>Double terminator</span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>3</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>3O</span></p> </td> <td width=101 style='width:60.85pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center;line-height:150%'><span lang=EN-US>pSB1AK3</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>3189 3318 445</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>V1010</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>AK</span></p> </td> </tr> <tr style='mso-yfti-irow:3'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><em><b style='mso-bidi-font-weight:normal'><span lang=EN-US>BBa_b0034</span></b></em><b style='mso-bidi-font-weight:normal'><span lang=EN-US><o:p></o:p></span></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>RBS</span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>1</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>3O</span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>pSB1A2</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>2079 2091 250</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>V1004</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>A</span></p> </td> </tr> <tr style='mso-yfti-irow:4'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><em><b style='mso-bidi-font-weight:normal'><span lang=EN-US>BBa_I14032</span></b></em><b style='mso-bidi-font-weight:normal'><span lang=EN-US><o:p></o:p></span></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>constitutive promoter</span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>2</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>17K</span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>pSB2K3</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>4425 4462 353</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>V1006</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>K</span></p> </td> </tr> <tr style='mso-yfti-irow:5'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><em><b style='mso-bidi-font-weight:normal'><span lang=EN-US>BBa_E0020</span></b></em><b style='mso-bidi-font-weight:normal'><span lang=EN-US><o:p></o:p></span></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>E-CFP</span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>1</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><st1:metricconverter ProductID="7C" w:st="on"><span lang=EN-US>7C</span></st1:metricconverter></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>pSB1A2</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>2079 2802 961</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>V1001</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>A</span></p> </td> </tr> <tr style='mso-yfti-irow:6'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><em><b style='mso-bidi-font-weight:normal'><span lang=EN-US>BBa_E0030</span></b></em><b style='mso-bidi-font-weight:normal'><span lang=EN-US><o:p></o:p></span></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>E-YFP</span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>1</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>7G</span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>pSB1AK3</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>3189 3912 1039</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>V1003</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>AK</span></p> </td> </tr> <tr style='mso-yfti-irow:7'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><em><b style='mso-bidi-font-weight:normal'><span lang=EN-US>BBa_E0044</span></b></em><b style='mso-bidi-font-weight:normal'><span lang=EN-US><o:p></o:p></span></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>GFP</span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>1</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>5B</span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>pSB1A3</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>2157 2916 1075</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>V1001</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>A</span></p> </td> </tr> <tr style='mso-yfti-irow:8'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><em><b style='mso-bidi-font-weight:normal'><span lang=EN-US>BBa_J06504</span></b></em><b style='mso-bidi-font-weight:normal'><span lang=EN-US><o:p></o:p></span></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span class=SpellE><span lang=EN-US>mRFP</span></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>2</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>7N</span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>pSB1A2</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>2079 2793 952</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>V1009</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>A</span></p> </td> </tr> <tr style='mso-yfti-irow:9'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>BBa_I12007<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>lambda <span class=SpellE>Prm</span> promoter activated by <span class=SpellE>cI</span><o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>2<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>15K<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>pSB2K3<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>4425 4507 398<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>V1006<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>K<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:10'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>BBa_C0051<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span class=SpellE><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2;mso-bidi-font-weight: bold'>cI</span></span><span lang=EN-US style='color:#C0504D;mso-themecolor: accent2;mso-bidi-font-weight:bold'> repressor from E. coli phage lambda (+LVA)</span><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'><o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>1<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>5G<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>pSB1A2<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>2079 2829 988<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>V1004<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#C0504D;mso-themecolor:accent2'>A<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:11'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>BBa_R0062<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>promoter activated by <span class=SpellE>HSL&LuxR</span><o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>1<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>9G<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>pSB1A2<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>2079 2134 293<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>V1004<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>A<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:12'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>BBa_C0062<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span class=SpellE><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>luxR</span></span><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'> repressor/activator<o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>1<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>7A<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>pSB1A2<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>2079 2835 994<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>V1004<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>A<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:13'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>BBa_C0061<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>Synthesizes 3OC<sub>6</sub>HSL, which binds to <span class=SpellE>LuxR</span><o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>1<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>5O<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>pSB1A2<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>2079 2697 856<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>V1004<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#76923C;mso-themecolor:accent3;mso-themeshade:191'>A<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:14'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>BBa_R0079<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191;mso-bidi-font-weight: bold'>Promoter (<span class=SpellE>LasR</span> & PAI regulated)</span><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'><o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>1<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>19N<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>pSB1A2<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>2079 2236 395<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>V1001<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>A<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:15'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>BBa_C0179<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span class=SpellE><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>LasR</span></span><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'> activator<o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>1<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>3J<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>pSB1A2<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>2079 2802 961<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>V1003<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>A<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:16'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>BBa_C0078<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span class=SpellE><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>autoinducer</span></span><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'> <span class=SpellE>synthetase</span> for PAI<o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>2<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>7I<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>pSB2K3<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>4425 5067 958<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>V1004<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#5F497A;mso-themecolor:accent4;mso-themeshade:191'>K<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:17'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>BBa_R0080<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A; mso-themecolor:accent6;mso-themeshade:191;mso-bidi-font-weight:bold'>Promoter (<span class=SpellE>AraC</span> regulated)</span><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'><o:p></o:p></span></p> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'><o:p> </o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>1<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>21L<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>pSB1A2<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>2079 2228 387<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>V1001<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>A<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:18;height:6.75pt'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>BBa_C0080<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span class=SpellE><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>araC</span></span><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'> <span class=SpellE>arabinose</span> <span class=SpellE>operon</span> regulatory protein<o:p></o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>2<o:p></o:p></span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>7M<o:p></o:p></span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>pSB2K3<o:p></o:p></span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>4425 5340 1231<o:p></o:p></span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>V1004<o:p></o:p></span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US style='color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191'>K<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:19;mso-yfti-lastrow:yes;height:6.75pt'> <td width=125 valign=top style='width:74.85pt;border:solid windowtext 1.0pt; border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:normal'><span lang=EN-US>BBa_I1051<o:p></o:p></span></i></b></p> </td> <td width=153 valign=top style='width:92.0pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p align=center style='text-align:center'><span class=SpellE><span lang=EN-US style='mso-bidi-font-weight:bold'>Lux</span></span><span lang=EN-US style='mso-bidi-font-weight:bold'> cassette right promoter</span></p> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US><o:p> </o:p></span></p> </td> <td width=58 valign=top style='width:34.8pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>1</span></p> </td> <td width=67 valign=top style='width:40.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>18P</span></p> </td> <td width=101 valign=top style='width:60.85pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>pSB1A2</span></p> </td> <td width=158 valign=top style='width:94.8pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>2079 2147 306</span></p> </td> <td width=83 valign=top style='width:49.5pt;border-top:none;border-left:none; border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>-</span></p> </td> <td width=59 valign=top style='width:35.15pt;border-top:none;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:6.75pt'> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US>A</span></p> </td> </tr> </table> <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US><o:p> </o:p></span></p> </div> </body> </html>
