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-	Biobricking of crtN	+	Biobricking of crtN and bch-gene cluster
	GenecrtN		GenecrtN

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Revision as of 04:23, 27 October 2007

Biobricking of crtN and bch-gene cluster

GenecrtN gene

PCR of crtN on Heliobacillus mobilis G/C content on annealing region

crtN-F 40% BglII CrtN-R 50% BamHI and XhoI

Primers

crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’

crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’