Berkeley LBL/Laina Notebook
From 2007.igem.org
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Biobricking of crtN and bch-gene cluster | Biobricking of crtN and bch-gene cluster | ||
- | + | Gene: | |
- | [[gene]] | + | crtN [[gene]] |
PCR of crtN on Heliobacillus mobilis | PCR of crtN on Heliobacillus mobilis |
Revision as of 04:23, 27 October 2007
Biobricking of crtN and bch-gene cluster
Gene: crtN gene
PCR of crtN on Heliobacillus mobilis G/C content on annealing region
crtN-F 40% BglII CrtN-R 50% BamHI and XhoI
Primers
crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’
crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’