Berkeley LBL/Laina Notebook

From 2007.igem.org

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Biobricking of crtN and bch-gene cluster
Biobricking of crtN and bch-gene cluster
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GenecrtN
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Gene:
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[[gene]]
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crtN [[gene]]
PCR of crtN on Heliobacillus mobilis  
PCR of crtN on Heliobacillus mobilis  

Revision as of 04:23, 27 October 2007

Biobricking of crtN and bch-gene cluster

Gene: crtN gene

PCR of crtN on Heliobacillus mobilis G/C content on annealing region

crtN-F 40% BglII CrtN-R 50% BamHI and XhoI

Primers

crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’

crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’