Berkeley LBL/Laina Notebook

(Difference between revisions)

Revision as of 04:32, 27 October 2007

Biobricking of bch-gene cluster and crtN gene.

Gene:

[[crtNgene]]

crtN

PCR of crtN on Heliobacillus mobilis G/C content on annealing region

crtN-F 40% BglII CrtN-R 50% BamHI and XhoI

Primers

crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’

crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’ bch B|3'ttaGGATCCccgttcgccttggtttgacttact

Revision as of 04:28, 27 October 2007 (view source) Silvapy (Talk \| contribs) ← Older edit		Revision as of 04:32, 27 October 2007 (view source) Silvapy (Talk \| contribs) Newer edit →
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	crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’		crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’
		+	bch B\|3'ttaGGATCCccgttcgccttggtttgacttact