Berkeley LBL/Laina Notebook
From 2007.igem.org
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'''Materials''' | '''Materials''' | ||
'''Methods''' | '''Methods''' | ||
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+ | Biobricking of bch-gene cluster and crtN gene. | ||
Gene: | Gene: | ||
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'''Results''' | '''Results''' | ||
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Revision as of 05:02, 27 October 2007
Materials
Methods
Biobricking of bch-gene cluster and crtN gene.
Gene:
bchB | bchE | bchI | bchL | bchM | bchN | [[crtNgene]] |
crtN
PCR of crtN on Heliobacillus mobilis G/C content on annealing region
crtN-F 40% BglII CrtN-R 50% BamHI and XhoI
Primers
crtN-F 3’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt5’
crtN-R 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’
bch B 3'ttaGGATCCccgttcgccttggtttgacttact5'
Results
Discussion