NYMU Taipei/Lab Notes/2007 12 12
From 2007.igem.org
< NYMU Taipei/Lab Notes(Difference between revisions)
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* pOmpC + EYFP culture | * pOmpC + EYFP culture | ||
** 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin (after 20hr, OD600 is 0.004) | ** 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin (after 20hr, OD600 is 0.004) | ||
+ | * pLacIQ + OmpRBS + GFP | ||
+ | ** re-culture pLacIQ in liquid medium (Kan+) | ||
+ | ** re-culture GFP in liquid medium () | ||
+ | ** re-culture EYFP in liquid medium () |
Latest revision as of 04:22, 13 December 2007
- pOmpC + EYFP culture
- 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin (after 20hr, OD600 is 0.004)
- pLacIQ + OmpRBS + GFP
- re-culture pLacIQ in liquid medium (Kan+)
- re-culture GFP in liquid medium ()
- re-culture EYFP in liquid medium ()