Template:BerkiGEM2007 HannahConstructionFiles
From 2007.igem.org
(Difference between revisions)
Revision as of 21:23, 28 June 2007
PCR HNX-F and HNX-R on H-Nox (567bp, BglII/XhoI)
Sub into pBca1144 ( BglII/XhoI, 2054+910 L)
Product is pBca1144-H-NOX
HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG
HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA